| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75.0 | 18.75 | 0.25 |
| L-Alanine | 89.0 | 4.45 | 0.049999997 |
| L-Arginine hydrochloride | 211.0 | 147.5 | 0.69905216 |
| L-Asparagine-H2O | 150.0 | 7.5 | 0.05 |
| L-Aspartic acid | 133.0 | 6.65 | 0.05 |
| L-Cysteine hydrochloride-H2O | 176.0 | 17.56 | 0.09977272 |
| L-Cystine 2HCl | 313.0 | 31.29 | 0.09996805 |
| L-Glutamic Acid | 147.0 | 7.35 | 0.05 |
| L-Glutamine | 146.0 | 365.0 | 2.5 |
| L-Histidine hydrochloride-H2O | 210.0 | 31.48 | 0.14990476 |
| L-Isoleucine | 131.0 | 54.47 | 0.41580153 |
| L-Leucine | 131.0 | 59.05 | 0.45076334 |
| L-Lysine hydrochloride | 183.0 | 91.25 | 0.4986339 |
| L-Methionine | 149.0 | 17.24 | 0.11570469 |
| L-Phenylalanine | 165.0 | 35.48 | 0.2150303 |
| L-Proline | 115.0 | 17.25 | 0.15 |
| L-Serine | 105.0 | 26.25 | 0.25 |
| L-Threonine | 119.0 | 53.45 | 0.44915968 |
| L-Tryptophan | 204.0 | 9.02 | 0.04421569 |
| L-Tyrosine disodium salt dihydrate | 261.0 | 55.79 | 0.21375479 |
| L-Valine | 117.0 | 52.85 | 0.4517094 |
| Vitamins | |||
| Biotin | 244.0 | 0.0035 | 1.4344263E-5 |
| Choline chloride | 140.0 | 8.98 | 0.06414285 |
| D-Calcium pantothenate | 477.0 | 2.24 | 0.0046960167 |
| Folic Acid | 441.0 | 2.65 | 0.0060090707 |
| Niacinamide | 122.0 | 2.02 | 0.016557377 |
| Pyridoxine hydrochloride | 206.0 | 2.013 | 0.009771844 |
| Riboflavin | 376.0 | 0.219 | 5.824468E-4 |
| Thiamine hydrochloride | 337.0 | 2.17 | 0.0064391694 |
| Vitamin B12 | 1355.0 | 0.68 | 5.0184503E-4 |
| i-Inositol | 180.0 | 12.6 | 0.07 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111.0 | 116.6 | 1.0504504 |
| Cupric sulfate (CuSO4-5H2O) | 250.0 | 0.0013 | 5.2E-6 |
| Ferric Nitrate (Fe(NO3)3"9H2O) | 404.0 | 0.05 | 1.2376238E-4 |
| Ferric sulfate (FeSO4-7H2O) | 278.0 | 0.417 | 0.0015 |
| Magnesium Chloride (anhydrous) | 95.0 | 28.64 | 0.30147368 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120.0 | 48.84 | 0.407 |
| Potassium Chloride (KCl) | 75.0 | 311.8 | 4.1573334 |
| Sodium Bicarbonate (NaHCO3) | 84.0 | 2438.0 | 29.02381 |
| Sodium Chloride (NaCl) | 58.0 | 6995.5 | 120.61207 |
| Sodium Phosphate dibasic (Na2HPO4) anhydrous | 142.0 | 71.02 | 0.50014085 |
| Sodium Phosphate monobasic (NaH2PO4-H2O) | 138.0 | 62.5 | 0.45289856 |
| Zinc sulfate (ZnSO4-7H2O) | 288.0 | 0.432 | 0.0015 |
| Other Components | |||
| D-Glucose (Dextrose) | 180.0 | 3151.0 | 17.505556 |
| Hypoxanthine Na | 159.0 | 2.39 | 0.015031448 |
| Linoleic Acid | 280.0 | 0.042 | 1.4999999E-4 |
| Lipoic Acid | 206.0 | 0.105 | 5.097087E-4 |
| Phenol Red | 376.4 | 8.1 | 0.021519661 |
| Putrescine 2HCl | 161.0 | 0.081 | 5.031056E-4 |
| Sodium Pyruvate | 110.0 | 55.0 | 0.5 |
| Thymidine | 242.0 | 0.365 | 0.0015082645 |
Reference:
1. Dulbecco, R. and Freeman, G., (1959) Plaque formation by the polyoma virus. Virology 8:396.
2. Ham, R.G., (1965) Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.
3. Morton, H.J. (1970) A survey of commercially available tissue culture media. In Vitro 6(2):89.
4. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:185.
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