After careful consideration, we’ve decided to discontinue the Neon Transfection System effective July 31, 2023.
Protect your valuable research time and samples with a compact electroporation machine designed and engineered for performance, speed, simplicity, and flexibility. The combination of the Invitrogen Neon NxT Electroporation System’s proprietary pipette tip technology, simplified workflow, and flexibility helps reduce sample loss, lowers contamination risk, saves you steps, and can put you in control of your experiments.
Benefits of the Neon NxT Electroporation System
- Proven transfection efficiency and cell viability—transfect challenging cell lines with high efficiency and high cell viability
- Flexibility when you need it—precisely optimize electroporation parameters for different cell types, cell densities, payloads, and electroporation protocols.
- Enhanced genome editing efficiency—optimized buffer for improved delivery of CRISPR molecules
- Save valuable research time—transfect cells quickly with an easy three step workflow, a single buffer kit and intuitive user interface
- Preserve your precious samples—minimize sample transfer loss and contamination risk
Order the Neon NxT Electroporation System
Watch step-by-step video on how to use the Neon NxT Electroporation System
Achieve high transfection efficiency and high cell viability in a broad range of cell lines using this transfection system
The Neon NxT Electroporation System is an electroporation instrument that offers up to 90% transfection and gene-editing efficiency in extremely difficult-to-transfect cells, including immune, primary, and stem cells. Over 150 cell lines have been tested with optimized ready-to-use conditions for efficiency and viability.
Search the Transfection protocols and citations database for optimized electroporation protocols on different cell lines.
Figure 1. Transfection efficiency with the Neon NxT Electroporation System. Cells were transfected with GFP plasmid DNA or GFP mRNA in 10 μL or 100 μL electroporation reactions. Transfection efficiency is reported as the percentage of GFP-positive cells (n = 3). Note: Naive T cells were only electroporated in 100 μL reactions.
Figure 2. Cell viability following electroporation on the Neon NxT system. Cells were transfected with GFP plasmid DNA or GFP mRNA in 10 μL or 100 μL electroporation reactions. Transfected cells were stained with Invitrogen SYTO Red Dead Cell Stain and assessed for viability on the Invitrogen ATTUNE NxT Flow Cytometer. Cell viability (%) is reported as the mean of 3 measurements. Note: Naive T cells were only electroporated in 100 μL reactions.
Figure 3. Performance of Neon NxT Resuspension Genome Editing Buffer in CRISPR-Cas9–based genome editing experiments. Cells were electroporated in 10 μL or 100 μL reactions. (A) GFP donor DNA knock-in efficiency reported as the percentage of GFP-positive cells. (B) Viability of cells after GFP donor DNA knock-in. (C) Knockout efficiency reported as percentage of initial GFP-positive cells not expressing GFP after electroporation. (D) Post-electroporation viability of knockout cells.
Enhancing genome editing efficiency
Genome editing holds great promise in the pursuit of a greater understanding of human health and disease. A critical, yet challenging step in the genome editing workflow is the effective delivery of CRISPR ribonucleoprotein (RNP), DNA and RNA molecules into the cell line of choice—also known as transfection. As demonstrated above, electroporation is the most widely used delivery method for its ability to achieve high transfection efficiency, even with hard-to-transfect cells.
When used with the Neon NxT Electroporation System, our gene editing reagents and the Neon NxT Resuspension Genome Editing Buffer can help improve gene editing performance with specific payloads like CRISPR Cas9 for knockout or knock-in experiments with mammalian cells including primary, stem, and difficult-to-transfect cells (Figure 3).
Explore our comprehensive suite of gene editing workflow solutions at thermofisher.com/geneediting
Why is the Neon NxT Electroporation System a better alternative to the electroporation cuvette?
Figure 4. Neon NxT pipette tip advantages compared to standard electroporation cuvette. The proprietary biologically compatible (A) Neon NxT tip presents a proven capillary electroporation technology that has important advantages over (B) electroporation cuvettes.
Unlike standard cuvette-based electroporation chambers, the Neon NxT electroporation system uses a proprietary biologically compatible pipette tip chamber that generates a more uniform electric field. This design allows for a better maintenance of physiological conditions resulting in high cell survival compared to conventional electroporation.†
- Creates a more uniform electric field
- Maintains a stable pH throughout the electroporation chamber
- Generates minimal heat
- Forms fewer ions
- Reduces shear force experienced by cells
Precisely optimize electroporation parameters for different cell types, cell densities, payloads, and electroporation protocols
Customizable electroporation parameters
With the Neon NxT Electroporation System, you can precisely control the parameters that matter most for your experiment without wasting precious time adjusting those that do not. This transfection system allows you to modify:
- Pulse voltage
- Pulse width
- Number of pulses
- Cell type
- Buffer type
- Payload type
Versatility for different payloads, cell types, and cell densities
Deliver DNA, RNA, or protein to a wide variety of mammalian cells, with the flexibility to transfect 1 x 104 to 1 x 107 cells per reaction.
Plan with ease using the TransfectionLab cloud-based app
Invitrogen TransfectionLab, a cloud-based app found on Thermo Fisher Connect, can automatically generate step-by-step guide tailored for each electroporation experiment, starting from the design stage. You can remotely set up multiple plate layouts for up to 384 samples, and any protocol or plate layout saved in the app can be accessed directly on the connected Neon NxT system.
Transfect cells quickly with an easy three-step workflow, a single buffer kit, and an intuitive user interface
Simplified electroporation workflow
Easy to follow and execute, the Neon NxT Electroporation System streamlined workflow requires minimal training and improves consistency and reproducibility.
Using this transfection system, the electroporation process is simple: first, take up cells and plasmid mix into the Neon NxT pipette tip. Second, plug into the pipette holder, and press Electroporate. Finally, pipette the transfected cells back into your culture vessel. As the transfection occurs in the pipette tip, the transfection is completed in just 3 simple steps: Aspirate, Electroporate, Dispense.
| Step 1 |
|---|
AspiratePrepare cells by suspending them in a Neon NxT buffer. Load cells and payload mix suspended inNeon NxT buffer into the Neon NxT pipette tip. |
| Step 2 |
Electroporate Apply electrical pulses to cells to deliver the payload in the specialized buffer. |
| Step 3 |
DispenseRestore growth conditions to allow the transfected cells to recover. |
| Step 4 |
Analyze cells Evaluate: e.g., gene expression, genome editing, silencing, and cell line development |
Shorter processing time using this electroporation system
In addition to simplifying the workflow, our proprietary electroporation technology shortens the overall process to 10–15 minutes, compared to a conventional electroporation system as the transfection occurs in the Neon NxT pipette tip.†
A single buffer kit for many mammalian cell types
Avoid the hassle of searching for a buffer kit that will work with your cell line. We have simplified the process with one buffer kit that is compatible with over 150 mammalian cell lines. We recommend using Invitrogen Neon NxT Resuspension R Buffer at voltages below 1,900 V and Invitrogen Neon NxT Resuspension T Buffer at higher voltages.
Time-saving plate setup
Rather than adjusting electroporation parameters between samples with Quick Start, set up an entire plate beforehand using the Neon NxT Electroporation System’s intuitive user interface. Monitor progress on the screen as you electroporate each of your samples, and electroporate up to 24 samples in less than 20 minutes.
Minimize sample transfer loss and contamination risk of valuable cells with unique and compact electroporation instrument design
Minimal sample transfer loss
Your samples are precious, but some volume is inevitably lost during transfer to and from a conventional electroporation cuvette. Electroporation on the Neon NxT Electroporation System occurs within the pipette tip, helping to minimize sample loss and the amount of shear force experienced by cells.
Minimal sample contamination
The compact footprint of the Neon NxT system, including the pulse generator and pipette station, makes it small enough to fit in most biosafety cabinets (BSCs). Your samples always stay within the aseptic area while using the instrument, helping reduce the risk of contaminating precious cells. The Neon NxT system also has a new cable management feature that makes using it in the BSC hassle-free.
Figure 5. Dimensions of the Neon NxT Electroporation System. The Neon NxT pulse generator (9.5 in. W x 7.6 in. H x 9.9 in. D) and Neon NxT pipette station (4.9 in. W x 11.5 in. H x 5.9 in. D) fit inside most BSCs, which helps minimize sample contamination.
Specification comparison between the Neon NxT Electroporation System and the Neon Transfection System
| Specification |  Neon NxT Electroporation System |  Neon Transfection System |
|---|---|---|
| Electroporation Volume | 10 µL or 100 µL | 10 µL or 100 µL |
| Electroporation buffer volume* | 2 mL | 3 mL |
| Tip attachment | ClipTip technology | Friction |
| Electroporation pulses | 1-10 | 1-10 |
| Pulse duration | 1-100 ms | 1-100 ms |
| Pulse voltage | 500-2,500 V | 500-2,500 V |
| Arc detection | Yes | No |
| Cloud connectivity | Yes | No |
| Pulse generator dimensions** | 9.5 x 7.6 x 9.9 in. (W x H x D) 11. 9 lb (5.4 kg) | 9.5 x 8.9 x 13.6 in. (W x H x D) 13. 8 lb (6.25 kg) |
| Cable management feature*** | Yes | No |
| Touch display | 8-inch capacitive touchscreen | 7-inch touchscreen |
| Electrical rating | 100-240 VAC, 270 W | 100-240 C, 150 W |
*The buffer tube of the Neon NxT Electroporation System has a 2 mL level indicator.
**The Neon NxT pulse generator can be moved into or out of a typical BSC without removing the sash.
***Excess cable length can be secured behind the Neon NxT system with the attachable cable organizer.
The same technology and performance as the Neon Transfection System
Figure 6. Performance of the Neon NxT Electroporation System and the Neon Transfection System. Performance was evaluated by transfecting different mammalian cell lines with GFP plasmid DNA or GFP mRNA. (A) GFP plasmid DNA transfection efficiency reported as the percentage of GFP-positive cells. (B) Viability of cells after transfection with GFP plasmid DNA. (C) GFP mRNA transfection efficiency reported as the percentage of GFP-positive cells. (D) Viability of cells after transfection with GFP mRNA.
† Kim JA, Cho K, Shin MS, et al. (2008) A novel electroporation method using a capillary and wire-type electrode. Biosens Bioelectron 23(9):1353–1360.
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