LinkSeq Blood Genotyping

Fast, easy, comprehensive molecular antigen typing using real-time PCR

Thermo Scientific LinkSēq real-time PCR molecular antigen typing kits overcome the major challenges of molecular typing by increasing laboratory productivity, reducing turnaround time, and predicting phenotypes for many antigens given the common variants for those antigens.

Why choose LinkSēq for blood genotyping?

Simplicity

Fast workflow with little hands-on effort

10 minutes hands-on time—simply add your DNA to the pre-aliquoted buffer/enzyme mix, add to the tray, seal, and you are done

Easy data analysis via SureTyper Blood

Automatically calls genotype and predicts phenotypes based on ISBT allele database and proprietary algorithm—in seconds
Simple data drill-down tools
Easy to use, intuitive interface

Flexibility

Broad instrument compatibility
High throughput to a single sample

Confidence

Accurate real-time PCR

Allele-specific amplification combined with SYBR Green to detect PCR products without chips or gels

Robust contamination protection

Samples are handled only once and then sealed in reaction trays
Increased sample protection as amplified PCR products remain sealed

Fast turnaround

DNA sample to results in 90 minutes
Just 10 minutes of hands-on time

LinkSēq portfolio for blood genotyping

For fast, easy, and comprehensive molecular genotyping, we introduce the LinkSeq portfolio for blood genotyping: LinkSeq ABO, and LinkSeq HPA typing kits.

LinkSēq – fast workflow with minimal hands-on time

LinkSēq ABO genotyping and subgrouping

ABO alleles encode the enzyme Glycosyltransferase. Many A subgroups involve a weakly expressed A antigen, which can cause discordant serological reactions in forward and reverse typing. Our ABO products offers a better solution to research these alleles by separating A1 from non-A1, B from B3, Bweak and Bel, and much more.

Coverage Includes:

A, B, AB, cisAB, O
A subgroups: A1 or non-A1, ie, A2, A3, Aweak, Ael or Am
B subgroups: B, B3, Bweak or Bel
O subgroups: O1, O2
Possible Bombay phenotype

View LinkSēq ABO 384 typing kits

Example of ABO results provided in the SureTyper Blood Software

Generate clear results in seconds

View raw data

LinkSēq HPA genotyping

Human Platelet Antigens (HPA) are glycoproteins expressed on platelet membranes. Our HPA assays are designed to target both the a and b alleles for HPA-1 through HPA-11, and HPA-15.

Coverage includes:

HPA-1 through HPA-11
HPA-15

View LinkSēq HPA typing kits

Example of HPA results provided in the SureTyper Blood Software