Resources for the Original Attune Cytometer | Thermo Fisher Scientific

photo of the original Attune Flow Cytometer

First-generation Attune Flow Cytometer support

Find technical resources for the original first-generation Invitrogen Attune Flow Cytometer, including manuals, reagent selection tables, software download information, and ordering information for consumables and accessories for your instrument.

Expand your research capabilities with the next-generation Attune Flow Cytometers

Manuals and publications

Attune Flow Cytometer Software Demo

Reagent selection guide for the original Attune Flow Cytometer

Blue/violet instrument configuration

Excitation laser	Default filter (nm)	Filter range (nm)	Fluorophores	Other fluorescent reagents
Violet 405 nm	450/40	430–470	Alexa Fluor 405 Pacific Blue	PO-PRO-1 DyeCycle Violet Fixable Violet Dead Cell Stain CellTrace Violet Calcein Violet SYTOX Blue FxCycle Violet Click-iT Pacific Blue
	522/31	507–537	Qdot 525 Horizon V500	Fixable Aqua Dead Cell Stain F2N12S (apoptotic)
	603/48	579–627	Pacific Orange Qdot 605	Fixable Yellow Dead Cell Stain F2N12S (live)
Blue 488 nm	530/30	515–545	Alexa Fluor 488 Fluorescein	Calcein Fluo-3/Fluo-4 TO-PRO-1 iodide CFSE GFP JC-1/DiOC₂(3) SYTOX Green DyeCycle Green Rhodamine 123 YO-PRO-1 iodide Fixable Green Dead Cell Stain Click-iT Alexa Fluor 488
	574/26	561–587	PE	PI Fura Red AM cell permeant DyeCycle Orange JC-1/DiOC₂(3) pHrodo Phagocytosis Particle Labeling Kit SNARF (low pH) isomers SYTOX Orange Dead cell stain
	640LP	>640	PE-Alexa Fluor 610 PerCP PE-Alexa Fluor 700 PE-Cy5.5 TRI-COLOR (TC) PE-Cy5 PerCP-Cy5.5 PE-Cy7 PE-PE-Alexa Fluor 750 Qdot 655 Qdot 705 Qdot 800	JC-1/DiOC₂(3) Fixable Red Dead Cell Stain 7-AAD SNARF (high pH) isomers SYTOX AADvanced Dead Cell Stain DyeCycle Ruby

Blue/red instrument configuration

Excitation laser	Default filter (nm)	Filter range (nm)	Fluorophores	Other fluorescent reagents
Blue 488 nm	530/30	515–545	Alexa Fluor 488 Fluorescein	Calcein Fluo-3/Fluo-4 TO-PRO-1 iodide CFSE GFP JC-1/DiOC₂(3) SYTOX Green DyeCycle Green Rhodamine 123 YO-PRO-1 iodide Fixable Green Dead Cell Stain Click-iT Alexa Fluor 488
	574/26	561–587	PE	PI Fura Red AM cell permeant DyeCycle Orange JC-1/DiOC₂(3) pHrodo Phagocytosis Particle Labeling Kit SNARF (low pH) isomers SYTOX Orange Dead cell stain
	690/50	665–715	Qdot 705 PerCP TRI-COLOR PE-Cy5 PerCP-Cy5.5 PE-Cy5.5	Vybrant Dye Cycle Ruby
	780/60	750–810	PE-Alexa Fluor 750 Qdot 800 PE-Cy 7	Vybrant Dye Cycle Ruby
Red 637 nm	660/20	650–670	Allophycocyanin (APC) Alexa Fluor 647	Fixable Far Red Dead Cell Stain Click-iT Alexa Fluor 647 FxCycle Far Red SYTOX Red Dead cell stain TO-PRO-3
Red 637 nm	780/60	750–810	APC-Alexa Fluor 750 APC-Cy 7	Fixable Near-IR Dead Cell Stain Vybrant Dye Cycle Ruby

Attune Autosampler

The Invitrogen Attune Autosampler is compatible with the original Attune Flow Cytometer and gives you the flexibility of automated multi-sample processing, offering:

Broad compatibility—compatible with many different plate formats, including 96-well, 384-well, and deep-well plates.
Mixing by aspiration—mixing sample by aspiration instead of shaking ensures homogeneity of the sample and helps maintains cell viability
Intelligent probe—intelligent probe design minimizes clogging and carryover (<0.5%) and prevents damage to the instrument
Auto cleaning—performs automated cleaning when the instrument is shutting down
Consistent data—Designed to provide minimal variation regardless of sampling method (tube vs. plate) and collection rate

See how the CytKick and CytKick Max autosamplers work with the Attune CytPix and Attune NxT flow cytometers by requesting an in-lab demo from one of our trained representatives.

Request a demo Request a quote

Attune cytometric software

The software for the original Attune Flow Cytometer can be downloaded without licensing fees and can be added to any desktop or laptop computer at your institution without any additional costs. Results can be easily analyzed at your convenience at your own computer, allowing the next user to run experiments on the Attune cytometer. Before initiating the download process, use the table below to determine which software version your system uses.

	Attune Cytometer WITHOUT Autosampler		Attune Cytometer WITH Autosampler

Purchase date of Attune Cytometer	BEFORE January 1st, 2013	AFTER January 1st, 2013	—
Software version	v1.2.5 *	v2.1.0 †	v2.1.0 †
Operating system	Windows™ XP	64-bit Windows 7	64-bit Windows 7
* To get the benefits of the v2.1.0 software requires the purchase of an Upgrade Kit; or you can upgrade to v2.1.0 for free with the purchase of an Attune Autosampler. † Version 2.1.0 requires a 64-bit Windows 7 computer. 8 GB RAM with minimal 500 GB storage are recommended.

Why should I upgrade to v2.1.0?

If you purchased your Attune Cytometer without an Autosampler before January 1, 2013, you may be running software v1.2.5 on a Windows XP system. To get the benefits of the v2.1.0 software requires the purchase of an upgrade kit; or you can upgrade to v2.1.0 for free with the purchase of an Attune Autosampler.

Better performance—responsiveness of software v2.1.0 is dramatically improved over v1.2.5
Faster analysis of samples—save time with batch processing, ability to select multiple objects, and easier experiment setup and data analysis
Sample recovery—no other syringe-driven system offers the ability to recover unused samples
Increased functionality—additional functionality beyond supporting the use of 96- and 384-well plates

The Attune Computer Upgrade Kit (Cat. No. 4481653) includes:

A Dell™ 64-bit computer with the Windows 7 operating system
Attune Cytometric Software v2.1 and v2.1 Documentation Kit
A visit by trained service personnel to assist in the transfer of data from the existing computer system

Download the Upgrade Kit Flyer

There are two ways to purchase the upgrade kit:

Standalone kit: Attune Software (v2.1.0) and Computer Upgrade Kit, including on-site installation and data transfer (Cat. No. 4481653); or
Kit + Service Contract: 1-year service contract and the upgrade kit together (35% savings compared to purchasing separately). Contact your sales rep to request a quote.

Resources for the Original Attune Flow Cytometer