Mechanisms of protein degradation
Learn about the different mechanisms of protein degradation including cellular functions each pathway mediates and which assays can be used to evaluate protein degradation.
Protein degradation has recently emerged as a selective and potentially effective approach to identify new drug targets, by reducing specific cellular proteins—using targeted protein degradation. Understanding the mechanism by which proteins are degraded and identifying the tools available to target individual proteins will allow for the development and advancement of treatments for various diseases and disorders.
The Protein Degradation Resource Center includes the latest information and research tools to assist you in evaluating protein degradation by providing product offerings and solutions to your everyday protein degradation questions.
Foundations of protein degradation
Protein degradation using the ubiquitin-proteasome pathway
Learn about protein degradation through the ubiquitin-proteasome pathway (UPP) and tools to assess if your protein of interest is undergoing degradation.
Targeted protein degradation using proteolysis-targeted chimeras
Learn about targeted protein degradation using proteolysis-targeted chimeras, and tools used for targeted protein degradation (TPD).
Solutions for your protein degradation questions
Answer your protein degradation questions with our protein analysis products. Thermo Fisher Scientific offers high-quality reagents, assays, and instruments for experimental needs.
Complex formation
Does my probe target my protein of interest and E3 ligase to properly form a ternary complex?
A chimera or small molecule probe must have available binding sites for both the E3 ligase and protein of interest. Determine if the ternary complex is formed and initiated targeted protein degradation of the protein of interest.
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Polyubiquitination
Is our protein of interest being polyubiquitinated?
Polyubiquitination tags the protein of interest for degradation by the proteasome. Determine the state of ubiquitin modification of the protein of interest in cell or tissue lysates and confirm the protein has been tagged for degradation.
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Protein degradation
Was my protein of interest degraded?
As an alternative to gene deletion methods, use targeted protein degradation to modulate expression of the protein of interest. Determine if the protein of interest was targeted for degradation.
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Biological assessment
How is targeted protein degradation affecting my host cells?
Evaluate any off-target effects of protein degradation. Host cells can be biologically assessed to determine the impact on cell viability and function.
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Research tools for protein degradation
Protein assay selection tool
Compare and choose the best protein assay based on sample type, assay time, read-out (colorimetric or fluorescent), and compatibility to detergents or reducing agents.
Protein gel selection tool
Compare and choose protein gels for SDS-PAGE or native PAGE based on sample type, volume, gel chemistry, separation range, gel size, well format, compatible gel tanks, and other features.
BlotBuilder western blot selection tool
Identify western blotting solutions specifically optimized for your protein and detection needs.
Antibody search tool
Easily search our extensive protfolio of high-quality primary and secondary antibodies validated and cited for western blotting, ELISA, flow cytometry, and other applications.
Fluorescence SpectraViewer
Compare the spectra and spectral compatibility of multiple fluorophores.
Flow Cytometry Panel Builder
Choose the optimal combination of fluorochromes for your flow cytometry experiments with this simplified guided tool.
The learning place
Learn about the latest methods and technologies for your protein research including protocols, troubleshooting tips, webinars, and articles.
Resources & support
Style Sheet for Global Design System
For Research Use Only. Not for use in diagnostic procedures.