Our capabilities enable us to provide complete support for all types of polyclonal antibody production from peptide design, synthesis, and carrier protein conjugation to animal immunization, serum collection, titer analysis, and final antibody purification. We specialize in antigen design and generation of custom peptide antibodies and monospecific peptide antibodies to highly discrete epitopes. We can provide protein expression services or immunize with recombinant proteins or protein fragments provided by you to generate antibodies in rabbits, chickens, goats, guinea pigs, mice, or rats.
Our capabilities enable us to provide complete support for all steps of mouse monoclonal antibody development from antigen preparation to the four phases of monoclonal development: 1) Animal Immunization, 2) Fusion, 3) Subcloning, and 4) Antibody Production. The final deliverables of this process are the purified monoclonal antibody and the hybridoma cell line, and our capable project manager and scientists will support you personally throughout the process.
Our recombinant antibodies are rabbit monoclonal antibodies developed by cloning immunogen-specific antibody genes into mammalian expression vectors. This is performed by immunizing rabbits with a supplied or synthesized immunogen, cloning the immunogen-specific antibody genes into mammalian expression vectors, expressing the specific antibodies in mammalian cell lines, and screening the generated antibodies for specificity. Recombinant rabbit antibodies can be produced large scale with a high degree of lot-to-lot consistency. Our recombinant rabbit antibodies have been proven to work in most applications including ELISA, flow cytometry, chromatin IP, western blot, immunocytochemistry (ICC), and immunohistochemistry (IHC).
We offer the following custom antibody purifications. We can also customize the purification method based on your specific needs.
- Antigen-specific affinity purification
- PTM-specific affinity purification
- Protein A/G purification
- Chicken IgY purification (from eggs)
We also provide integrated services for chemically labeling purified antibodies to create conjugated antibodies for use in nearly any assay system. Example conjugates include, but are not limited to:
