Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran.

Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran (Cat. no. L22460). MDCK cells labeled with the fluorescent dextran were exposed to pH-calibration buffers (pH 3.5, top panel; or pH 6.0, bottom panel) in the presence of nigericin (Cat. no. N1495) and monensin. Pseudocolored images were constructed from two emission images at 450 ± 33 nm and 510 ± 20 nm, both excited at 365 ± 8 nm. (In these images, fluorescence near pH 3 has been pseudocolored blue and fluorescence near pH 6 pseudocolored yellow-green.) (Chem Biol 6, 411 (1999))

Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran (Cat. no. L22460). MDCK cells labeled with the fluorescent dextran were exposed to pH-calibration buffers (pH 3.5, top panel; or pH 6.0, bottom panel) in the presence of nigericin (Cat. no. N1495) and monensin. Pseudocolored images were constructed from two emission images at 450 ± 33 nm and 510 ± 20 nm, both excited at 365 ± 8 nm. (In these images, fluorescence near pH 3 has been pseudocolored blue and fluorescence near pH 6 pseudocolored yellow-green.) (Chem Biol 6, 411 (1999))

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