Transformed mouse fibroblasts (CRE BAG 2 cells).

lacZ mRNA in transformed mouse fibroblasts (CRE BAG 2 cells) are hybridized with a singly biotinylated, complementary oligonucleotide. Hybrids were then detected by incubation with a streptavidin–alkaline phosphatase conjugate in combination with the ELF 97 alkaline phosphatase substrate, both of which are provided in our ELF® 97 mRNA In Situ Hybridization Kit (Cat. No. E6604, E6605). Cells were counterstained with DAPI (Cat. No. D1306, D3571, D21490) and photographed using a longpass optical filter appropriate for DAPI.

<i>lacZ</i> mRNA in transformed mouse fibroblasts (CRE BAG 2 cells) are hybridized with a singly biotinylated, complementary oligonucleotide. Hybrids were then detected by incubation with a streptavidin–alkaline phosphatase conjugate in combination with the ELF 97 alkaline phosphatase substrate, both of which are provided in our ELF® 97 mRNA <i>In Situ</i> Hybridization Kit (Cat. No. E6604, E6605). Cells were counterstained with DAPI (Cat. No. D1306, D3571, D21490) and photographed using a longpass optical filter appropriate for DAPI.

Related Products

Related Images

Prostate carcinoma. Go ›

Zebrafish brain. ELF 97 Endogenous Phosphatase Detection Kit. Go ›

1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›

Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›

Simultaneous detection of expression of five genes in a whole mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes Go ›

Labeled paint probes hybridized to human metaphase chromosomes Go ›