Gibco

Rat M-CSF Recombinant Protein, PeproTech®

Product Details

400-28-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 19 publications 19 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Rat

Published species

Mouse, Rat

Expression System

E. coli

Amino acid sequence

MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP

Molecular weight

36.2 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is ≤ 5.0 ng/ml, corresponding to a specific activity of ≥ 2 x 10^5 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Rat M-CSF is a 36.2 kDa homodimeric protein consisting of two 155 amino acid polypeptide subunits.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Glomerulonephritis and autoimmune vasculitis are independent of P2RX7 but may depend on alternative inflammasome pathways.

The Journal of pathology

Prendecki M,McAdoo SP,Turner-Stokes T,Garcia-Diaz A,Orriss I,Woollard KJ,Behmoaras J,Cook HT,Unwin R,Pusey CD,Aitman TJ,Tam FW

Fri Jul 01 00:00:00 EDT 2022

Interleukin-20 Acts as a Promotor of Osteoclastogenesis and Orthodontic Tooth Movement.

Stem cells international

Liu Y,Ai Y,Sun X,Meng B,Chen X,Wu D,Gan L,Yang B,Fu C,Wu Y,Cao Y

Sat Apr 23 00:00:00 EDT 2022

Sodium Lactate Accelerates M2 Macrophage Polarization and Improves Cardiac Function after Myocardial Infarction in Mice.

Cardiovascular therapeutics

Zhang J,Huang F,Chen L,Li G,Lei W,Zhao J,Liao Y,Li Y,Li C,Chen M

Wed Jul 21 00:00:00 EDT 2021

Exosomes Derived from M2 Macrophages Exert a Therapeutic Effect via Inhibition of the PI3K/AKT/mTOR Pathway in Rats with Knee Osteoarthritic.

BioMed research international

Da-Wa ZX,Jun M,Chao-Zheng L,Sen-Lin Y,Chuan L,De-Chun L,Zu-Nan D,Hong-Tao Z,Shu-Qing W,Xian-Wei P,Wenbo L,Ke-Wen L

Thu Jan 20 00:00:00 EST 2022

C-type lectin receptors Mcl and Mincle control development of multiple sclerosis-like neuroinflammation.

The Journal of clinical investigation

N'diaye M,Brauner S,Flytzani S,Kular L,Warnecke A,Adzemovic MZ,Piket E,Min JH,Edwards W,Mela F,Choi HY,Magg V,James T,Linden M,Reichardt HM,Daws MR,van Horssen J,Kockum I,Harris RA,Olsson T,Guerreiro-Cacais AO,Jagodic M

Mon Feb 03 00:00:00 EST 2020

Blockade of adrenergic β-receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair in vivo.

Cell proliferation

Wu H,Song Y,Li J,Lei X,Zhang S,Gao Y,Cheng P,Liu B,Miao S,Bi L,Yang L,Pei G

Wed Jan 01 00:00:00 EST 2020

Beneficial effects of IL-4 and IL-6 on rat neonatal target cardiac cells.

Scientific reports

Zogbi C,Oliveira NC,Levy D,Bydlowski SP,Bassaneze V,Neri EA,Krieger JE

Thu Jul 23 00:00:00 EDT 2020

Astrocyte-to-astrocyte contact and a positive feedback loop of growth factor signaling regulate astrocyte maturation.

Glia

Li J,Khankan RR,Caneda C,Godoy MI,Haney MS,Krawczyk MC,Bassik MC,Sloan SA,Zhang Y

Thu Aug 01 00:00:00 EDT 2019

Anti-inflammatory effect of Tauroursodeoxycholic acid in RAW 264.7 macrophages, Bone marrow-derived macrophages, BV2 microglial cells, and spinal cord injury.

Scientific reports

Kim SJ,Ko WK,Jo MJ,Arai Y,Choi H,Kumar H,Han IB,Sohn S

Fri Feb 16 00:00:00 EST 2018

LncRNA LINC00311 Promotes the Proliferation and Differentiation of Osteoclasts in Osteoporotic Rats Through the Notch Signaling Pathway by Targeting DLL3.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Wang Y,Luo TB,Liu L,Cui ZQ

400-28 was used in Cell Culture to suggest that LINC00311 induces proliferation and inhibits apoptosis of osteoclasts via the regulation of the Notch signaling pathway by inhibiting DLL3 expression, ultimately demonstrating that LINC00311 and its target gene DLL3 may serve as independent factors in cases of osteoporosis.

Fri Aug 17 00:00:00 EDT 2018

Fibrinogen Activates BMP Signaling in Oligodendrocyte Progenitor Cells and Inhibits Remyelination after Vascular Damage.

Neuron

Petersen MA,Ryu JK,Chang KJ,Etxeberria A,Bardehle S,Mendiola AS,Kamau-Devers W,Fancy SPJ,Thor A,Bushong EA,Baeza-Raja B,Syme CA,Wu MD,Rios Coronado PE,Meyer-Franke A,Yahn S,Pous L,Lee JK,Schachtrup C,Lassmann H,Huang EJ,Han MH,Absinta M,Reich DS,Ellisman MH,Rowitch DH,Chan JR,Akassoglou K

Wed Dec 06 00:00:00 EST 2017

FcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells.

Frontiers in immunology

Pan YG,Yu YL,Lin CC,Lanier LL,Chu CL

400-28 was used in Cell Culture to further explore the role of the tyrosine-based activation motif-containing adaptors DAP12 and FcεRI γ-chain in C-type lectin receptor responses.

Wed Nov 20 00:00:00 EST 2019

VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism.

Nature communications

Li J,Diao B,Guo S,Huang X,Yang C,Feng Z,Yan W,Ning Q,Zheng L,Chen Y,Wu Y

Mon Nov 06 00:00:00 EST 2017

Selective A2A receptor antagonist prevents microglia-mediated neuroinflammation and protects retinal ganglion cells from high intraocular pressure-induced transient ischemic injury.

Translational research : the journal of laboratory and clinical medicine

Madeira MH,Boia R,Elvas F,Martins T,Cunha RA,Ambrósio AF,Santiago AR

Tue Mar 01 00:00:00 EST 2016

Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.

PloS one

Lobato-Pascual A,Saether PC,Dahle MK,Gaustad P,Dissen E,Fossum S,Daws MR

Tue Sep 10 00:00:00 EDT 2013

Effects and interaction of icariin, curculigoside, and berberine in er-xian decoction, a traditional chinese medicinal formula, on osteoclastic bone resorption.

Evidence-based complementary and alternative medicine : eCAM

Xue L,Jiao L,Wang Y,Nie Y,Han T,Jiang Y,Rahman K,Zhang Q,Qin L

Tue Dec 18 00:00:00 EST 2012

NADPH oxidase is internalized by clathrin-coated pits and localizes to a Rab27A/B GTPase-regulated secretory compartment in activated macrophages.

The Journal of biological chemistry

Ejlerskov P,Christensen DP,Beyaie D,Burritt JB,Paclet MH,Gorlach A,van Deurs B,Vilhardt F

Fri Feb 10 00:00:00 EST 2012

Constitutive expression of TNF-related activation-induced cytokine (TRANCE)/receptor activating NF-κB ligand (RANK)-L by rat plasmacytoid dendritic cells.

PloS one

Anjubault T,Martin J,Hubert FX,Chauvin C,Heymann D,Josien R

Mon Aug 20 00:00:00 EDT 2012

R-Ras and Rac GTPase cross-talk regulates hematopoietic progenitor cell migration, homing, and mobilization.

The Journal of biological chemistry

Shang X,Cancelas JA,Li L,Guo F,Liu W,Johnson JF,Ficker A,Daria D,Geiger H,Ratner N,Zheng Y

Fri Jul 08 00:00:00 EDT 2011

The angiogenic response of the aorta to injury and inflammatory cytokines requires macrophages.

Journal of immunology (Baltimore, Md. : 1950)

Gelati M,Aplin AC,Fogel E,Smith KD,Nicosia RF

Wed Oct 15 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CSF-1; H-MCSF; M-MCSF; macrophage colony-stimulating factor 1; MCSF; MGC31930

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Entrez Gene ID: (Rat) 78965