Gibco

Rat IFN-gamma Recombinant Protein, PeproTech®

Product Details

400-20-100UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 32 publications 32 publications

Miscellaneous PubMed (Misc)

View 6 publications 6 publications

Product Specifications

Species

Rat

Published species

Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC

Molecular weight

15.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to inhibit the proliferation of mouse WEHI-279 cells. The expected-ED50 for this effect is 0.1-1.0 ng/ml

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Rat IFN-gamma is a 15.6 kDa protein containing 135 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Clostridioides difficile toxin B alone and with pro-inflammatory cytokines induces apoptosis in enteric glial cells by activating three different signalling pathways mediated by caspases, calpains and cathepsin B.

Cellular and molecular life sciences : CMLS

Fettucciari K,Marguerie F,Fruganti A,Marchegiani A,Spaterna A,Brancorsini S,Marconi P,Bassotti G

400-20 was used in Sample Preparation (FC/FACS) to investigate effects of the virulence factors produced during Clostridioides difficile infection.

Fri Jul 22 00:00:00 EDT 2022

Interferon-γ enhances the therapeutic effect of mesenchymal stem cells on experimental renal fibrosis.

Scientific reports

Kanai R,Nakashima A,Doi S,Kimura T,Yoshida K,Maeda S,Ishiuchi N,Yamada Y,Ike T,Doi T,Kato Y,Masaki T

Wed Jan 13 00:00:00 EST 2021

Antiviral Inflammation during Early Pregnancy Reduces Placental and Fetal Growth Trajectories.

Journal of immunology (Baltimore, Md. : 1950)

Baines KJ,Rampersaud AM,Hillier DM,Jeyarajah MJ,Grafham GK,Eastabrook G,Lacefield JC,Renaud SJ

Sat Feb 01 00:00:00 EST 2020

Neuronal gamma oscillations and activity-dependent potassium transients remain regular after depletion of microglia in postnatal cortex tissue.

Journal of neuroscience research

Lewen A,Ta TT,Cesetti T,Hollnagel JO,Papageorgiou IE,Chausse B,Kann O

Thu Oct 01 00:00:00 EDT 2020

Beneficial effects of IL-4 and IL-6 on rat neonatal target cardiac cells.

Scientific reports

Zogbi C,Oliveira NC,Levy D,Bydlowski SP,Bassaneze V,Neri EA,Krieger JE

Thu Jul 23 00:00:00 EDT 2020

Journal of cellular physiology

Liu M,Yin L,Li W,Hu J,Wang H,Ye B,Tang Y,Huang C

Thu Aug 01 00:00:00 EDT 2019

Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats.

Scientific reports

Stanisavljević S,Čepić A,Bojić S,Veljović K,Mihajlović S,Đedović N,Jevtić B,Momčilović M,Lazarević M,Mostarica Stojković M,Miljković Đ,Golić N

Tue Jan 29 00:00:00 EST 2019

Oligodendrocyte precursor cells present antigen and are cytotoxic targets in inflammatory demyelination.

Nature communications

Kirby L,Jin J,Cardona JG,Smith MD,Martin KA,Wang J,Strasburger H,Herbst L,Alexis M,Karnell J,Davidson T,Dutta R,Goverman J,Bergles D,Calabresi PA

Thu Aug 29 00:00:00 EDT 2019

BMSCs pre-treatment ameliorates inflammation-related tissue destruction in LPS-induced rat DIC model.

Cell death & disease

Wang B,Wu S,Ma Z,Wang T,Yang C

Wed Oct 03 00:00:00 EDT 2018

Bidirectional modulation between infiltrating CD3⁺ T-lymphocytes and astrocytes in the spinal cord drives the development of allodynia in monoarthritic rats.

Scientific reports

Zhou YL,Zhou SZ,Li HL,Hu ML,Li H,Guo QH,Deng XM,Zhang YQ,Xu H

Mon Jan 08 00:00:00 EST 2018

The distinct roles of mesenchymal stem cells in the initial and progressive stage of hepatocarcinoma.

Cell death & disease

Zong C,Zhang H,Yang X,Gao L,Hou J,Ye F,Jiang J,Yang Y,Li R,Han Z,Wei L

Thu Mar 01 00:00:00 EST 2018

Mechanisms and modulation of microvesicle uptake in a model of alveolar cell communication.

The Journal of biological chemistry

Schneider DJ,Speth JM,Penke LR,Wettlaufer SH,Swanson JA,Peters-Golden M

Fri Dec 22 00:00:00 EST 2017

TLR4-activated microglia require IFN-γ to induce severe neuronal dysfunction and death in situ.

Proceedings of the National Academy of Sciences of the United States of America

Papageorgiou IE,Lewen A,Galow LV,Cesetti T,Scheffel J,Regen T,Hanisch UK,Kann O

Tue Jan 05 00:00:00 EST 2016

Transcellular delivery of vesicular SOCS proteins from macrophages to epithelial cells blunts inflammatory signaling.

The Journal of experimental medicine

Bourdonnay E,Zasłona Z,Penke LR,Speth JM,Schneider DJ,Przybranowski S,Swanson JA,Mancuso P,Freeman CM,Curtis JL,Peters-Golden M

Mon May 04 00:00:00 EDT 2015

Estradiol promotes M1-like macrophage activation through cadherin-11 to aggravate temporomandibular joint inflammation in rats.

Journal of immunology (Baltimore, Md. : 1950)

Kou XX,Li CS,He DQ,Wang XD,Hao T,Meng Z,Zhou YH,Gan YH

Sun Mar 15 00:00:00 EDT 2015

PMC, a potent hydrophilic α-tocopherol derivative, inhibits NF-κB activation via PP2A but not IκBα-dependent signals in vascular smooth muscle cells.

Journal of cellular and molecular medicine

Hsieh CY,Hsiao G,Hsu MJ,Wang YH,Sheu JR

Tue Jul 01 00:00:00 EDT 2014

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) signaling and cell death in the immature central nervous system after hypoxia-ischemia and inflammation.

The Journal of biological chemistry

Kichev A,Rousset CI,Baburamani AA,Levison SW,Wood TL,Gressens P,Thornton C,Hagberg H

Fri Mar 28 00:00:00 EDT 2014

Nitric oxide induces ataxia telangiectasia mutated (ATM) protein-dependent γH2AX protein formation in pancreatic β cells.

The Journal of biological chemistry

Oleson BJ,Broniowska KA,Schreiber KH,Tarakanova VL,Corbett JA

Fri Apr 18 00:00:00 EDT 2014

Keratan sulfate expression in microglia is diminished in the spinal cord in experimental autoimmune neuritis.

Cell death & disease

Matsui H,Ohgomori T,Natori T,Miyamoto K,Kusunoki S,Sakamoto K,Ishiguro N,Imagama S,Kadomatsu K

Thu Dec 05 00:00:00 EST 2013

NADPH oxidase is internalized by clathrin-coated pits and localizes to a Rab27A/B GTPase-regulated secretory compartment in activated macrophages.

The Journal of biological chemistry

Ejlerskov P,Christensen DP,Beyaie D,Burritt JB,Paclet MH,Gorlach A,van Deurs B,Vilhardt F

Fri Feb 10 00:00:00 EST 2012

COUP-TFII controls mouse pancreatic β-cell mass through GLP-1-β-catenin signaling pathways.

PloS one

Boutant M,Ramos OH,Tourrel-Cuzin C,Movassat J,Ilias A,Vallois D,Planchais J,Pégorier JP,Schuit F,Petit PX,Bossard P,Maedler K,Grapin-Botton A,Vasseur-Cognet M

Mon Jun 04 00:00:00 EDT 2012

Cannabidiol protects oligodendrocyte progenitor cells from inflammation-induced apoptosis by attenuating endoplasmic reticulum stress.

Cell death & disease

Mecha M,Torrao AS,Mestre L,Carrillo-Salinas FJ,Mechoulam R,Guaza C

Thu Jun 28 00:00:00 EDT 2012

Rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.

PloS one

Jaubert AM,Penot G,Niang F,Durant S,Forest C

Thu Jan 10 00:00:00 EST 2013

Nicotinic acid inhibits progression of atherosclerosis in mice through its receptor GPR109A expressed by immune cells.

The Journal of clinical investigation

Lukasova M,Malaval C,Gille A,Kero J,Offermanns S

Tue Mar 01 00:00:00 EST 2011

Andrographolide enhances nuclear factor-kappaB subunit p65 Ser536 dephosphorylation through activation of protein phosphatase 2A in vascular smooth muscle cells.

The Journal of biological chemistry

Hsieh CY,Hsu MJ,Hsiao G,Wang YH,Huang CW,Chen SW,Jayakumar T,Chiu PT,Chiu YH,Sheu JR

Fri Feb 25 00:00:00 EST 2011

Interleukin-27 and interferon-gamma are involved in regulation of autoimmune arthritis.

The Journal of biological chemistry

Rajaiah R,Puttabyatappa M,Polumuri SK,Moudgil KD

Fri Jan 28 00:00:00 EST 2011

Mouse CD8alpha+ DCs and human BDCA3+ DCs are major producers of IFN-lambda in response to poly IC.

The Journal of experimental medicine

Lauterbach H,Bathke B,Gilles S,Traidl-Hoffmann C,Luber CA,Fejer G,Freudenberg MA,Davey GM,Vremec D,Kallies A,Wu L,Shortman K,Chaplin P,Suter M,O'Keeffe M,Hochrein H

Mon Nov 22 00:00:00 EST 2010

Glyconanoparticles allow pre-symptomatic in vivo imaging of brain disease.

Proceedings of the National Academy of Sciences of the United States of America

van Kasteren SI,Campbell SJ,Serres S,Anthony DC,Sibson NR,Davis BG

Tue Jan 06 00:00:00 EST 2009

Effect of inflammatory cytokines on major histocompatibility complex expression and differentiation of human neural stem/progenitor cells.

Stem cells (Dayton, Ohio)

Johansson S,Price J,Modo M

Mon Sep 01 00:00:00 EDT 2008

IL-4-induced selective clearance of oligomeric beta-amyloid peptide(1-42) by rat primary type 2 microglia.

Journal of immunology (Baltimore, Md. : 1950)

Shimizu E,Kawahara K,Kajizono M,Sawada M,Nakayama H

Sat Nov 01 00:00:00 EDT 2008

Expression and regulation of major histocompatibility complex on neural stem cells and their lineages.

Stem cells and development

Yin L,Fu SL,Shi GY,Li Y,Jin JQ,Ma ZW,Lu PH

Fri Feb 01 00:00:00 EST 2008

Dendritic cells trigger tumor cell death by a nitric oxide-dependent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Nicolas A,Cathelin D,Larmonier N,Fraszczak J,Puig PE,Bouchot A,Bateman A,Solary E,Bonnotte B

Sun Jul 15 00:00:00 EDT 2007

C-Jun N-terminal kinase (JNK) regulation of iNOS expression in glial cells: predominant role of JNK1 isoform.

Antioxidants & redox signaling

Pawate S,Bhat NR

Tue Mar 27 00:00:00 EDT 2007

Distinct roles for the NF-kappaB1 and c-Rel transcription factors in the differentiation and survival of plasmacytoid and conventional dendritic cells activated by TLR-9 signals.

Blood

O'Keeffe M,Grumont RJ,Hochrein H,Fuchsberger M,Gugasyan R,Vremec D,Shortman K,Gerondakis S

Tue Nov 15 00:00:00 EST 2005

Falcarindiol impairs the expression of inducible nitric oxide synthase by abrogating the activation of IKK and JAK in rat primary astrocytes.

British journal of pharmacology

Shiao YJ,Lin YL,Sun YH,Chi CW,Chen CF,Wang CN

Sat Jan 01 00:00:00 EST 2005

Targeting experimental autoimmune encephalomyelitis lesions to a predetermined axonal tract system allows for refined behavioral testing in an animal model of multiple sclerosis.

The American journal of pathology

Kerschensteiner M,Stadelmann C,Buddeberg BS,Merkler D,Bareyre FM,Anthony DC,Linington C,Brück W,Schwab ME

Thu Apr 01 00:00:00 EST 2004

Beneficial autoimmunity to proinflammatory mediators restrains the consequences of self-destructive immunity.

Immunity

Wildbaum G,Nahir MA,Karin N

Sat Nov 01 00:00:00 EST 2003

Dendritic cell precursor populations of mouse blood: identification of the murine homologues of human blood plasmacytoid pre-DC2 and CD11c+ DC1 precursors.

Blood

O'Keeffe M,Hochrein H,Vremec D,Scott B,Hertzog P,Tatarczuch L,Shortman K

Sat Feb 15 00:00:00 EST 2003

Mouse plasmacytoid cells: long-lived cells, heterogeneous in surface phenotype and function, that differentiate into CD8(+) dendritic cells only after microbial stimulus.

The Journal of experimental medicine

O'Keeffe M,Hochrein H,Vremec D,Caminschi I,Miller JL,Anders EM,Wu L,Lahoud MH,Henri S,Scott B,Hertzog P,Tatarczuch L,Shortman K

Mon Nov 18 00:00:00 EST 2002

Pioglitazone enhances cytokine-induced apoptosis in vascular smooth muscle cells and reduces intimal hyperplasia.

Circulation

Aizawa Y,Kawabe J,Hasebe N,Takehara N,Kikuchi K

Tue Jul 24 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

Rat IFN-gamma Recombinant Protein, PeproTech®

Product Details

400-20-100UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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