Invitrogen

Mouse EphA2 His-tag Recombinant Protein

Product Details

RP-88230

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

Product Specifications

Species

Mouse

Expression System

HEK293 cells

Amino acid sequence

Mouse EPHA2, amino acids Met1-Asn535 (Accession # NP_034269.2) with a C-terminal His-tag

Tag

His-tag

Molecular weight

65 kDa

Class

Recombinant

Type

Protein

Purity

>98% as determined by SDS-PAGE.

Endotoxin concentration

<1.0 EU/µg

Activity

Measured by its binding ability in a functional ELISA. 1. Immobilized mouse EphA2 at 2 µg/ml (100 µL/well) can bind mouse EphrinA1 with a linear range of 0.16-20 ng/mL. 2. Immobilized mouse EphA2 at 2 µg/ml (100 µL/well) can bind human EphrinA1 with a linear range of 0.8-20 ng/mL.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.

Concentration is measured by UV.

Target Information

EPHA2 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. EPHA2 is overexpressed in many types of human cancers. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; Tyrosine-protein kinase receptor MPK-5; Tyrosine-protein kinase receptor SEK-2