Gibco

Human LIF, Animal-Free Recombinant Protein, PeproTech®

Human LIF, Animal-Free Protein in Functional Assay (Functional)

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Human LIF, Animal-Free Protein (AF-300-05-25UG) in Functional

Bioassay analysis of Human LIF, Animal-Free Recombinant Protein, PeproTech® (Product # AF-300-05-1MG).

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Product Details

AF-300-05-25UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human, Rat

Expression System

E. coli

Amino acid sequence

SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF

Molecular weight

19.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human LIF is a 19.7 kDa protein containing 180 amino acid residues, including three disulfide bonds.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo.

Cell

Tan T,Wu J,Si C,Dai S,Zhang Y,Sun N,Zhang E,Shao H,Si W,Yang P,Wang H,Chen Z,Zhu R,Kang Y,Hernandez-Benitez R,Martinez Martinez L,Nuñez Delicado E,Berggren WT,Schwarz M,Ai Z,Li T,Rodriguez Esteban C,Ji W,Niu Y,Izpisua Belmonte JC

Thu Apr 15 00:00:00 EDT 2021

Oncostatin M induces hyperalgesic priming and amplifies signaling of cAMP to ERK by RapGEF2 and PKA.

Journal of neurochemistry

Garza Carbajal A,Ebersberger A,Thiel A,Ferrari L,Acuna J,Brosig S,Isensee J,Moeller K,Siobal M,Rose-John S,Levine J,Schaible HG,Hucho T

AF-300-05 was used in Sample Preparation to conclude that priming induced by OSM uses a novel mechanism to enhance and prolong coupling of cAMP/PKA to ERK1/2 signaling without changing the overall pathway structure.

Tue Jun 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: cholinergic differentiation factor; D factor; differentiation inhibitory activity; differentiation-inducing factor; Differentiation-stimulating factor; hepatocyte-stimulating factor III; human interleukin in DA cells; Leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI