Invitrogen

gamma Tubulin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

3 References

View all (31) gamma Tubulin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

gamma Tubulin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

gamma Tubulin Antibody (PA5-34815) in ICC/IF

gamma Tubulin Polyclonal Antibody detects gamma Tubulin protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin Polyclonal Antibody (Product # PA5-34815) diluted at 1:1,000. Red: alpha Tubulin, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:500. Blue: Fluoroshield wit... View More

gamma Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

gamma Tubulin Antibody in Western Blot (WB)

gamma Tubulin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

PA5-34815

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:100-1:500

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 184 of gamma Tubulin (Uniprot ID#P23258)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2552167

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, Jurkat, Raji, NCI-H929, NIH3T3, rat kidney.

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (98%), Xenopus laevis (99%), Dog (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phosphoproteomic analysis of neoadjuvant breast cancer suggests that increased sensitivity to paclitaxel is driven by CDK4 and filamin A.

Nature communications

Mouron S,Bueno MJ,Lluch A,Manso L,Calvo I,Cortes J,Garcia-Saenz JA,Gil-Gil M,Martinez-Janez N,Apala JV,Caleiras E,Ximénez-Embún P,Muñoz J,Gonzalez-Cortijo L,Murillo R,Sánchez-Bayona R,Cejalvo JM,Gómez-López G,Fustero-Torre C,Sabroso-Lasa S,Malats N,Martinez M,Moreno A,Megias D,Malumbres M,Colomer R,Quintela-Fandino M

PA5-34815 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot, Sample Prep to find candidate biomarkers of paclitaxel sensitivity using phosphoproteomic screening of samples from HER2-negative female breast cancer receiving neoadjuvant paclitaxel.

Wed Dec 07 00:00:00 EST 2022

The PP1 regulator PPP1R2 coordinately regulates AURKA and PP1 to control centrosome phosphorylation and maintain central spindle architecture.

BMC molecular and cell biology

Bresch AM,Yerich N,Wang R,Sperry AO

PA5-34815 was used in Immunocytochemistry-immunofluorescence to reveal the involvement of protein phosphatase 1 regulatory subunit 2 in coordinating protein phosphatase 1 and AURKA activity during cytokinesis.

Wed Nov 25 00:00:00 EST 2020

The PP1 regulator PPP1R2 coordinately regulates AURKA and PP1 to control centrosome phosphorylation and maintain central spindle architecture.

BMC molecular and cell biology

Bresch AM,Yerich N,Wang R,Sperry AO

Wed Nov 25 00:00:00 EST 2020

A novel interaction between kinase activities in regulation of cilia formation.

BMC cell biology

DeVaul N,Koloustroubis K,Wang R,Sperry AO

PA5-34815 was used in Immunohistochemistry to demonstrate a complex interplay between Nek2 and AurA kinases in regulation of ciliation in ARPE-19 cells.

Wed Nov 15 00:00:00 EST 2017

Bioinformatics

Protein Aliases: centrosome marker; Gamma-1-tubulin; Gamma-2-tubulin; Gamma-tubulin complex component 1; GCP-1; Tubulin gamma-1 chain; Tubulin gamma-2 chain; tubulin, gamma 1; tubulin, gamma 2; tubulin, gamma polypeptide

View more View less

Gene Aliases: 1500010O08Rik; AI451582; AI503389; AI504772; CDCBM4; GCP-1; TUBG; TUBG1; TUBG2; TUBGCP1; Tubgl; zgc:55894

View more View less

UniProt ID: (Human) P23258, (Human) Q9NRH3, (Mouse) P83887, (Rat) P83888, (Mouse) Q8VCK3

View more View less

Entrez Gene ID: (Human) 7283, (Human) 27175, (Mouse) 103733, (Rat) 252921, (Rat) 680991, (Mouse) 103768, (Zebrafish) 393882

View more View less

Function(s)

GTPase activity structural constituent of cytoskeleton protein binding GTP binding structural molecule activity nucleotide binding tubulin

Process(es)

G2/M transition of mitotic cell cycle meiotic spindle organization microtubule cytoskeleton organization microtubule nucleation cytoplasmic microtubule organization microtubule-based process mitotic spindle organization regulation of mitotic nuclear division protein polymerization metabolic process mitotic sister chromatid segregation mitotic cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

gamma Tubulin Polyclonal Antibody

gamma Tubulin Antibody (PA5-34815) in ICC/IF

Product Details

PA5-34815

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!