Invitrogen

GRB2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

View all (21) GRB2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GRB2 Antibody in Immunocytochemistry (ICC/IF)

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GRB2 Antibody (PA5-17692) in ICC/IF

Immunofluorescence analysis of GRB2 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GRB2 Rabbit Polyclonal Antibody(Product # PA5-17692) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More

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GRB2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with GRB2 siRNA and decrease in signal intensity was observed in western blot application using Anti-GRB2 Polyclonal Antibody (Product # PA5-17692). {KD}

Product Details

PA5-17692

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to carboxy-terminal residues of human GRB2

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

217 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986571

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: abundant SRC homology; Adapter protein GRB2; ASH protein; epidermal growth factor receptor-binding protein GRB2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; OTTHUMP00000166097; OTTHUMP00000166098; Protein Ash; SH2/SH3 adapter GRB2

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Gene Aliases: AA408164; ASH; EGFRBP-GRB2; GRB2; Grb3-3; MST084; MSTP084; NCKAP2

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UniProt ID: (Human) P62993, (Mouse) Q60631, (Rat) P62994

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Entrez Gene ID: (Human) 2885, (Bovine) 535298, (Chinese hamster) 100769627, (Mouse) 14784, (Rat) 81504

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Function(s)

SH3/SH2 adaptor activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding neurotrophin TRKA receptor binding protein binding 1-phosphatidylinositol-3-kinase activity SH3 domain binding protein kinase binding protein phosphatase binding identical protein binding insulin receptor substrate binding poly(A) RNA binding ephrin receptor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity phosphotyrosine binding protein domain specific binding phosphoprotein binding scaffold/adaptor protein

Process(es)

MAPK cascade epidermal growth factor receptor signaling pathway Ras protein signal transduction cell-cell signaling axon guidance insulin receptor signaling pathway fibroblast growth factor receptor signaling pathway positive regulation of signal transduction regulation of phosphatidylinositol 3-kinase signaling viral process positive regulation of actin filament polymerization T cell costimulation receptor internalization phosphatidylinositol-3-phosphate biosynthetic process Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway signal transduction in response to DNA damage regulation of MAPK cascade positive regulation of GTPase activity phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling anatomical structure formation involved in morphogenesis leukocyte migration branching involved in labyrinthine layer morphogenesis cellular response to ionizing radiation positive regulation of reactive oxygen species metabolic process aging cell differentiation protein heterooligomerization

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GRB2 Polyclonal Antibody

GRB2 Antibody (PA5-17692) in ICC/IF

Product Details

PA5-17692

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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