Invitrogen

Androgen Receptor Polyclonal Antibody

Advanced Verification

IP-mass spectrometry

4 Published Figures
32 References
View all (100) Androgen Receptor antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA1-110

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
View 4 publications 4 publications

Immunohistochemistry (IHC)

-
View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100
-

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:100
-

Immunocytochemistry (ICC/IF)

Assay-dependent
View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-dependent
View 4 publications 4 publications

ChIP assay (ChIP)

-
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Hamster, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fusion protein containing residues 331-572 of human androgen receptor.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325809

Product Specific Information

PA1-110 detects androgen receptor (AR) from human, primate, rat, and mouse tissues. This antibody does not detect estrogen, progesterone, or glucocorticoid receptors.

PA1-110 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing AR from rat brain homogenate. Immunohistochemical staining of AR in rat brain with PA1-110 results in intense nuclear staining.

For fixation of frozen sections, 2% paraformaldehyde + 15% picric acid solution in phosphate buffer, pH 7.4 is recommended.

The PA1-110 immunogen is a fusion protein containing residues 331-572 of human androgen receptor.

Target Information

The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors. The receptor is more than 90 kDa and has three major functional domains: the N-terminal domain, DNA-binding domain, and the androgen-binding domain. The androgen receptor is a ligand-activated transcription factor that binds active testosterone (T) and dihydrotestosterone (DHT). Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Androgen Receptor is a phosphoprotein, and also regulates mitogen-activated protein kinase (MAP kinase). The inhibition of the MEK1/2 pathway correlates directly with a change in phosphorylation state of the androgen receptor. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease, and male infertility. Mutations in this gene are associated with complete androgen insensitivity (CAIS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: androgen nuclear receptor variant 2; Androgen receptor; androgen receptor (Testicular feminization), same as Tfm; androgen receptor splice variant 4b; AR-A; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; RP11-383C12.1; Variant AR45

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Gene Aliases: AIS; Andr; AR; AR8; AW320017; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM

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UniProt ID: (Human) P10275, (Rat) P15207, (Mouse) P19091

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Entrez Gene ID: (Human) 367, (Rat) 24208, (Mouse) 11835

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding androgen receptor activity receptor binding steroid binding androgen binding protein binding beta-catenin binding transcription factor binding zinc ion binding enzyme binding transcription regulatory region DNA binding protein dimerization activity ATPase binding protein domain specific binding sequence-specific DNA binding androgen receptor binding POU domain binding
Process(es)
transcription, DNA-templated transcription initiation from RNA polymerase II promoter transport signal transduction cell-cell signaling sex differentiation cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of gene expression cell growth androgen receptor signaling pathway intracellular receptor signaling pathway prostate gland development positive regulation of phosphorylation positive regulation of cell differentiation negative regulation of integrin biosynthetic process positive regulation of integrin biosynthetic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase III promoter positive regulation of NF-kappaB transcription factor activity protein oligomerization regulation of establishment of protein localization to plasma membrane negative regulation of extrinsic apoptotic signaling pathway in utero embryonic development regulation of systemic arterial blood pressure epithelial cell morphogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter spermatogenesis single fertilization male gonad development fertilization cellular process regulation of gene expression male somatic sex determination response to insulin positive regulation of intracellular estrogen receptor signaling pathway Leydig cell differentiation multicellular organism growth steroid hormone mediated signaling pathway positive regulation of MAPK cascade positive regulation of insulin-like growth factor receptor signaling pathway male sex differentiation reproductive structure development regulation of developmental growth organ formation male genitalia morphogenesis negative regulation of epithelial cell proliferation regulation of catalytic activity activation of prostate induction by androgen receptor signaling pathway morphogenesis of an epithelial fold lateral sprouting involved in mammary gland duct morphogenesis regulation of prostatic bud formation prostate gland growth prostate gland epithelium morphogenesis epithelial cell differentiation involved in prostate gland development tertiary branching involved in mammary gland duct morphogenesis mammary gland alveolus development reproductive system development cellular response to steroid hormone stimulus seminiferous tubule development
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