Invitrogen

CD155 Monoclonal Antibody (D171)

CD155 Antibody in Immunocytochemistry (ICC/IF)

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CD155 Antibody (MA5-13493) in ICC/IF

Immunofluorescent analysis of CD155 (green) showing staining in the membrane of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD155 monoclonal antibody (Product # MA5-13493) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conju... View More

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Product Details

MA5-13493

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10-1:100

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Flow Cytometry (Flow)

2 µg/test

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

D171

Immunogen

HeLa cells

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10978147

Product Specific Information

MA5-13493 targets CD155 in Western blot, FACS and ICC/IF applications and shows reactivity with Human, Mouse and Non-human primate samples.

The MA5-13493 immunogen is HeLa cells.

Target Information

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of clathrin-mediated endocytosis by knockdown of AP-2 leads to alterations in the plasma membrane proteome.

Traffic (Copenhagen, Denmark)

Tobys D,Kowalski LM,Cziudaj E,Müller S,Zentis P,Pach E,Zigrino P,Blaeske T,Höning S

MA5-13493 was used in Flow Cytometry to inhibit CME by depletion of AP-2 and explore the consequences for the plasma membrane proteome.

Fri Jan 01 00:00:00 EST 2021

Inhibition of clathrin-mediated endocytosis by knockdown of AP-2 leads to alterations in the plasma membrane proteome.

Traffic (Copenhagen, Denmark)

Tobys D,Kowalski LM,Cziudaj E,Müller S,Zentis P,Pach E,Zigrino P,Blaeske T,Höning S

MA5-13493 was used in Flow Cytometry to inhibit CME by depletion of AP-2 and explore the consequences for the plasma membrane proteome.

Fri Jan 01 00:00:00 EST 2021

High expression of soluble CD155 in estrogen receptor-negative breast cancer.

Breast cancer (Tokyo, Japan)

Iguchi-Manaka A,Okumura G,Ichioka E,Kiyomatsu H,Ikeda T,Bando H,Shibuya A,Shibuya K

MA5-13493 was used in an ELISA assay to investigate the expression of poliovirus receptor CD155 in human estrogen receptor-negative breast cancer.

Wed Jan 01 00:00:00 EST 2020

Innate immune activating ligand SUMOylation affects tumor cell recognition by NK cells.

Scientific reports

Zitti B,Molfetta R,Fionda C,Quatrini L,Stabile H,Lecce M,de Turris V,Ricciardi MR,Petrucci MT,Cippitelli M,Gismondi A,Santoni A,Paolini R

MA5-13493 was used in Proximity Ligation Assay to study the role of ubiquitin and ubiquitin-like modifiers in the regulation of ligand expression in tumour cells.

Tue Sep 05 00:00:00 EDT 2017

Inhibition of Necl-5 (CD155/PVR) reduces glioblastoma dispersal and decreases MMP-2 expression and activity.

Journal of neuro-oncology

Enloe BM,Jay DG

MA5-13493 was used in immunocytochemistry to investigate the effect of Necl-5 blockade on glioblastoma progression and specific gene expression

Fri Apr 01 00:00:00 EDT 2011

NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.

Molecular immunology

Verhoeven DH,de Hooge AS,Mooiman EC,Santos SJ,ten Dam MM,Gelderblom H,Melief CJ,Hogendoorn PC,Egeler RM,van Tol MJ,Schilham MW,Lankester AC

MA5-13493 was used in immunohistochemistry to investigate the effect of natural killer cells on Ewing sarcoma and its mechanism

Mon Sep 01 00:00:00 EDT 2008

CD155/PVR enhances glioma cell dispersal by regulating adhesion signaling and focal adhesion dynamics.

Cancer research

Sloan KE,Stewart JK,Treloar AF,Matthews RT,Jay DG

MA5-13493 was used in immunocytochemistry to investigate the effect of CD155/PVR on glioma cell adhesion

Thu Dec 01 00:00:00 EST 2005

Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome.

Journal of virology

Ohka S,Matsuda N,Tohyama K,Oda T,Morikawa M,Kuge S,Nomoto A

MA5-13493 was used in immunocytochemistry to study CD155-dependent endocytosis and retrograde axonal transport of polivirus-containg vessicles

Thu Jul 01 00:00:00 EDT 2004

Identification of secreted CD155 isoforms.

Biochemical and biophysical research communications

Baury B,Masson D,McDermott BM,Jarry A,Blottière HM,Blanchardie P,Laboisse CL,Lustenberger P,Racaniello VR,Denis MG

MA5-13493 was used in western blot to study secreted human CD155 isoforms

Fri Sep 12 00:00:00 EDT 2003

Overexpression of the CD155 gene in human colorectal carcinoma.

Gut

Masson D,Jarry A,Baury B,Blanchardie P,Laboisse C,Lustenberger P,Denis MG

MA5-13493 was used in Immunohistochemistry to show that the CD155 gene is overexpressed in colorectal carcinoma and that this overexpression begins at an early stage in tumorigenesis and continues to late stages.

Wed Aug 01 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: CD155; FLJ25946; NECL-5; nectin-like 5; Nectin-like protein 5; Poliovirus receptor; sCD112; soluble CD112; tumor-associated antigen 1

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Gene Aliases: 3830421F03Rik; CD155; D7Ertd458e; HVED; mE4; Necl-5; NECL5; PVR; PVS; Taa1; TAGE4

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UniProt ID: (Human) P15151

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Entrez Gene ID: (Human) 5817, (Mouse) 52118

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Function(s)

virus receptor activity receptor activity receptor binding protein binding protein homodimerization activity cell adhesion molecule binding

Process(es)

single organismal cell-cell adhesion cell migration viral entry into host cell positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules cell recognition adherens junction organization susceptibility to natural killer cell mediated cytotoxicity positive regulation of natural killer cell mediated cytotoxicity regulation of immune response susceptibility to T cell mediated cytotoxicity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD155 Monoclonal Antibody (D171)

CD155 Antibody (MA5-13493) in ICC/IF

Product Details

MA5-13493

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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