Invitrogen

HSP60 Monoclonal Antibody (2E1/53)

1 Published Figure
3 References
View all (97) HSP60 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA3-013

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-10 µg/mL
-

Immunocytochemistry (ICC/IF)

1:20-1:200
-

ELISA (ELISA)

1-10 µg/mL
-

Immunoprecipitation (IP)

2 µg
-

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Rabbit

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

2E1/53

Immunogen

Human placental HSP60.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325461

Product Specific Information

MA3-013 detects HSP60 from human, monkey, mouse, and rat samples. This antibody displays no other protein reactivity.

MA3-013 has been successfully used in Western blot, immunoprecipitation, immunohistochemistry, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects a single 58 kDa protein representing recombinant human HSP60.

The MA3-013 antigen is human placental HSP60. Epitope mapping studies using human HSP60 deletion mutants suggest that this antibody binds between amino acids 211-288.

Reconstitute with PBS.

Target Information

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; Chaperonin 60; CPN60; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein; Heat shock protein 60; heat shock protein 60 (liver); heat shock protein 65; Heat shock protein family D member 1; heat shock protein, 60 kDa; HSP; HSP-60; HSP-65; Hsp60s1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1

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Gene Aliases: 60kDa; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; Hspd1-30p; HuCHA60; SPG13

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UniProt ID: (Human) P10809, (Mouse) P63038, (Rat) P63039

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Entrez Gene ID: (Human) 3329, (Mouse) 15510, (Rat) 63868

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Function(s)
lipopolysaccharide binding p53 binding DNA replication origin binding single-stranded DNA binding double-stranded RNA binding protein binding ATP binding ATPase activity ubiquitin protein ligase binding poly(A) RNA binding unfolded protein binding chaperone binding nucleotide binding protease binding protein complex binding insulin binding protein heterodimerization activity misfolded protein binding
Process(es)
detection of misfolded protein B cell cytokine production MyD88-dependent toll-like receptor signaling pathway positive regulation of T cell mediated immune response to tumor cell protein folding activation of cysteine-type endopeptidase activity involved in apoptotic process response to unfolded protein positive regulation of interferon-alpha production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production protein refolding B cell proliferation T cell activation B cell activation positive regulation of macrophage activation positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of neuron apoptotic process isotype switching to IgG isotypes positive regulation of inflammatory response protein stabilization positive regulation of T cell activation chaperone mediated protein folding requiring cofactor 'de novo' protein folding response to cold viral process chaperone-mediated protein complex assembly protein maturation response to hypoxia response to heat response to organic substance response to organic cyclic compound response to lipopolysaccharide response to ATP response to cocaine response to drug response to hydrogen peroxide response to estrogen
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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