Invitrogen

IgE Monoclonal Antibody (Ige21), FITC, eBioscience™

FIGURE: 1 / 1

IgE Antibody (11-6986-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human Fc epsilon Receptor I alpha (FceR1) APC (Product # 17-5899-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human IgE FITC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

11-6986-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Ige21

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717244

Product Specific Information

Description: The monoclonal antibody IgE21 recognizes the immunoglobulin E class of antibodies. The natural levels of IgE in the serum are quite low, comprising only a small portion of the total immunoglobulin. IgE plays a role in allergy responses by binding to mast cells and basophils through the Fc epsilon receptor I which results in the release of histamine and other molecules. The IgE is produced by terminally differentiated plasma B cells and basophils. Like all immunoglobulins, IgE is expressed as a soluble molecule as well as a membrane bound form. IgE21 recognizes both forms.

Applications Reported: This Ige21 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Ige21 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Human Mast Cell Proteome Reveals Unique Lineage, Putative Functions, and Structural Basis for Cell Ablation.

Immunity

Plum T,Wang X,Rettel M,Krijgsveld J,Feyerabend TB,Rodewald HR

11-6986 was used in Flow cytometry/Cell sorting to identify a mast-cell-specific proteome signature, indicative of a unique lineage, only distantly related to other immune cell types, including innate immune cells.

Tue Feb 18 00:00:00 EST 2020

The mechanistic and functional profile of the therapeutic anti-IgE antibody ligelizumab differs from omalizumab.

Nature communications

Gasser P,Tarchevskaya SS,Guntern P,Brigger D,Ruppli R,Zbären N,Kleinboelting S,Heusser C,Jardetzky TS,Eggel A

11-6986 was used in Flow cytometry/Cell sorting to investigate and compare the molecular binding profile and functional mechanisms of action of the anti-IgE antibody ligelizumab and omalizumab.

Wed Jan 08 00:00:00 EST 2020

Genetic Architecture of Adaptive Immune System Identifies Key Immune Regulators.

Cell reports

Lagou V,Garcia-Perez JE,Smets I,Van Horebeek L,Vandebergh M,Chen L,Mallants K,Prezzemolo T,Hilven K,Humblet-Baron S,Moisse M,Van Damme P,Boeckxstaens G,Bowness P,Dubois B,Dooley J,Liston A,Goris A

11-6986-42 was used in Flow Cytometry to identify eight genome-wide significant associations explaining 6%-20% of variance in immune system regulation.

Tue Oct 16 00:00:00 EDT 2018

Structural basis of omalizumab therapy and omalizumab-mediated IgE exchange.

Nature communications

Pennington LF,Tarchevskaya S,Brigger D,Sathiyamoorthy K,Graham MT,Nadeau KC,Eggel A,Jardetzky TS

11-6986 was used in Flow cytometry/Cell sorting to discover a treatment blocking basophil activation and provide a novel approach to probe mechanisms underlying IgE hypersensitivity.

Thu May 19 00:00:00 EDT 2016

NADPH oxidase-independent formation of extracellular DNA traps by basophils.

Journal of immunology (Baltimore, Md. : 1950)

Morshed M,Hlushchuk R,Simon D,Walls AF,Obata-Ninomiya K,Karasuyama H,Djonov V,Eggel A,Kaufmann T,Simon HU,Yousefi S

11-6986 was used in Flow cytometry/Cell sorting to suggest that basophils exert direct innate immune effector functions in the extracellular space.

Sun Jun 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Immunoglobulin E; ImmunoglobulinE

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