Invitrogen

TRADD Polyclonal Antibody

TRADD Antibody in Immunocytochemistry (ICC/IF)

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TRADD Antibody (PA5-21595) in ICC/IF

Immunofluorescent analysis of TRADD in methanol-fixed HeLa cells using a TRADD polyclonal antibody (Product # PA5-21595) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

TRADD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-21595

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 1 and 43 of TRADD (Uniprot ID#Q15628)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.54 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_11152323

Product Specific Information

PA5-21595 targets TRADD in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.

The PA5-21595 immunogen is a synthetic peptide corresponding to a region within amino acids 1 and 43 of TRADD (Uniprot ID#Q15628).

Target Information

TRADD (Tumor necrosis factor receptor type 1-associated DEATH domain protein) is an important mediator of FAS and TNFR1 associated apoptotic signaling. The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12. TRADD acts by interacting with TRIP12 and leading to the disruption of the interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A. TRADD acts as an adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interation with FADD. Overexpression of TRADD leads to two major TNF-induced responses - apoptosis and activation of NF-kappa-B.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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