Invitrogen

TCR V alpha 12.1 Monoclonal Antibody (6D6.6)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

6 References

View all (2) TCR V alpha 12.1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

TCR V alpha 12.1 Antibody in Western Blot (WB)

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TCR V alpha 12.1 Antibody (TCR1764) in WB

Western blot analysis of TCR V alpha 12.1 was performed by loading 25 µg of Jurkat (lane 1), CEM (lane 2) and rat thymus (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a TCR V alpha 12.1 monoclonal antibody (Product # TCR1764) at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce EC... View More

TCR V alpha 12.1 Antibody in Flow Cytometry (Flow)

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Product Details

TCR1764

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Flow Cytometry (Flow)

1-2 µg/test

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-dependent

T-Cell Activation (TCA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6D6.6

Immunogen

Human TCR V alpha 12.1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.5% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223507

Target Information

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genetically modified human CD4(+) T cells can be evaluated in vivo without lethal graft-versus-host disease.

Immunology

Ali R,Babad J,Follenzi A,Gebe JA,Brehm MA,Nepom GT,Shultz LD,Greiner DL,DiLorenzo TP

TCR1764 was used in Flow cytometry/Cell sorting to modify human primary CD4(+) T cells by lentiviral transduction to express a human TCR that recognizes a pancreatic beta cell-derived peptide in the context of HLA-DR4.

Mon Aug 01 00:00:00 EDT 2016

Restricted autoantigen recognition associated with deletional and adaptive regulatory mechanisms.

Journal of immunology (Baltimore, Md. : 1950)

Gebe JA,Yue BB,Unrath KA,Falk BA,Nepom GT

TCR1764 was used in flow cytometry to investigate the mechanism for autoreactive T cells in autoimmune diabetes

Wed Jul 01 00:00:00 EDT 2009

Autoreactive human T-cell receptor initiates insulitis and impaired glucose tolerance in HLA DR4 transgenic mice.

Journal of autoimmunity

Gebe JA,Unrath KA,Yue BB,Miyake T,Falk BA,Nepom GT

TCR1764 was used in flow cytometry to study the role of autoreactive human T-cell receptor in insulitis and impairement of glucose tolerance

Sun Jun 01 00:00:00 EDT 2008

Cutting edge: size and diversity of CD4+CD25high Foxp3+ regulatory T cell repertoire in humans: evidence for similarities and partial overlapping with CD4+CD25- T cells.

Journal of immunology (Baltimore, Md. : 1950)

Fazilleau N,Bachelez H,Gougeon ML,Viguier M

TCR1764 was used in flow cytometry to characterize the CD4+CD25high Foxp3+ regulatory T cell repertoire

Sat Sep 15 00:00:00 EDT 2007

Identification of an enhancer agonist cytotoxic T lymphocyte peptide from human carcinoembryonic antigen.

Cancer research

Zaremba S,Barzaga E,Zhu M,Soares N,Tsang KY,Schlom J

TCR1764 was used in flow cytometry to identify a specific peptide from human carcinoembryonic antigen to enhance the immunogenicity of tumor cell self-antigens

Wed Oct 15 00:00:00 EDT 1997

Clonal V alpha 12.1+ T cell expansions in the peripheral blood of rheumatoid arthritis patients.

The Journal of experimental medicine

DerSimonian H,Sugita M,Glass DN,Maier AL,Weinblatt ME,Rème T,Brenner MB

TCR1764 was used in immunohistochemistry to investigate the increase of V alpha 12.1+, CD8+ T cells in rheumatoid arthritis patients

Tue Jun 01 00:00:00 EDT 1993

Bioinformatics

Protein Aliases: TCRVA; TCRVA 12.1; V alpha 12.1; V alpha-12.1 TCR

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TCR V alpha 12.1 Monoclonal Antibody (6D6.6)

TCR V alpha 12.1 Antibody (TCR1764) in WB

Product Details

TCR1764

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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