Invitrogen

TBK1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 Published Figures

5 References

View all (32) TBK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

TBK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

TBK1 Antibody (PA5-17478) in ICC/IF

Immunofluorescence analysis of TBK1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TBK1 Polyclonal Antibody (Product # PA5-17478) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # PA5-17478) at... View More

TBK1 Antibody in Immunohistochemistry (IHC)

TBK1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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TBK1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of the target protein. A loss of signal was observed for the target protein in the TBK1 KO cell line compared to the control cell line using an Anti-TBK1 Polyclonal Antibody (Product # PA5-17478). {KO}

Product Details

PA5-17478

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

1:100

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues near the carboxy-terminus of TBK1/NAK

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

174 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981817

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

TBK1 serine/threonine kinase is a member of the IKK family and is involved in NF-kappa B activation and IRF3 signaling. TBK1 is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-KB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-KB is associated with IKB proteins in the cell cytoplasm, which inhibit NF-KB activity. Phosphorylation of I-KB by IKB kinase (IKK) complex leads to degradation of I-KB and activation of NF-KB. The IKK complex contains IKKa, IKK beta, and IKKK. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-KB-activating kinase), and T2K. NAK/TBK1 activates IKK beta through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.

F1000Research

Alshafie W,Fotouhi M,Shlaifer I,Ayoubi R,Edwards AM,Durcan TM,McPherson PS,Laflamme C

PA5-17478 was used in Western Blotting to identify antibodies that appear specific for all three applications but invite the readers to interpret the present findings based on their own scientific expertise and use this report as a guide to select the most appropriate antibody for their specific needs.

Wed Dec 07 00:00:00 EST 2022

Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.

F1000Research

Alshafie W,Fotouhi M,Shlaifer I,Ayoubi R,Edwards AM,Durcan TM,McPherson PS,Laflamme C

Wed Dec 07 00:00:00 EST 2022

Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.

F1000Research

Alshafie W,Fotouhi M,Shlaifer I,Ayoubi R,Edwards AM,Durcan TM,McPherson PS,Laflamme C

Wed Dec 07 00:00:00 EST 2022

Bacterial Cyclic Dinucleotides and the cGAS-cGAMP-STING Pathway: A Role in Periodontitis?

Pathogens (Basel, Switzerland)

Elmanfi S,Yilmaz M,Ong WWS,Yeboah KS,Sintim HO,Gürsoy M,Könönen E,Gürsoy UK

Published figure using TBK1 polyclonal antibody (Product # PA5-17478) in Immunohistochemistry

Sun May 30 00:00:00 EDT 2021

Association of the TBK1 mutation p.Ile334Thr with frontotemporal dementia and literature review.

Molecular genetics & genomic medicine

Yu H,Yu W,Luo SS,Yang YJ,Liu FT,Zhang Y,Chen Y,Sun YM,Wu JJ

Published figure using TBK1 polyclonal antibody (Product # PA5-17478) in Western Blot

Fri Mar 01 00:00:00 EST 2019

Haploinsufficiency of TBK1 causes familial ALS and fronto-temporal dementia.

Nature neuroscience

Freischmidt A,Wieland T,Richter B,Ruf W,Schaeffer V,Müller K,Marroquin N,Nordin F,Hübers A,Weydt P,Pinto S,Press R,Millecamps S,Molko N,Bernard E,Desnuelle C,Soriani MH,Dorst J,Graf E,Nordström U,Feiler MS,Putz S,Boeckers TM,Meyer T,Winkler AS,Winkelman J,de Carvalho M,Thal DR,Otto M,Brännström T,Volk AE,Kursula P,Danzer KM,Lichtner P,Dikic I,Meitinger T,Ludolph AC,Strom TM,Andersen PM,Weishaupt JH

PA5-17478 was used in western blot to identify gene variants that contribute to amyotrophic lateral sclerosis

Fri May 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: FLJ11330; NF-kappa-B-activating kinase; NF-kB-activating kinase; serine/threonine protein kinase TBK1; Serine/threonine-protein kinase TBK1; T2K; TANK-binding kinase 1; tbk

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Gene Aliases: 1200008B05Rik; AI462036; AW048562; FTDALS4; NAK; T2K; TBK1

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UniProt ID: (Human) Q9UHD2, (Mouse) Q9WUN2

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Entrez Gene ID: (Human) 29110, (Mouse) 56480, (Rat) 299827

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Function(s)

nucleic acid binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding phosphoprotein binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase protein modifying enzyme

Process(es)

Performance Guarantee

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TBK1 Polyclonal Antibody

TBK1 Antibody (PA5-17478) in ICC/IF

Product Details

PA5-17478

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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