Invitrogen

Phospho-CREB (Ser133) Polyclonal Antibody

1 Published Figure
1 Reference
View all (63) CREB antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA1-4619

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
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Immunohistochemistry (IHC)

1:100-1:1000
-
Product Specifications

Species Reactivity

Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 of rat CREB1 conjugated to KLH
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2230004

Product Specific Information

This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate, rat, sheep, Xenopus and zebrafish based on 100% sequence homology.

This antibody is specific for the ~45 kDa CREB phosphorylated at Ser133 in Western blots of rat brain lysates. Immunolabeling can be blocked by lambda-phosphatase treatment.

Target Information

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cAMP response element binding protein 1; cAMP-responsive element-binding protein 1; CREB-1; Cyclic AMP-responsive element-binding protein 1; htCREB; MGC9284; OTTHUMP00000206661; OTTHUMP00000206662; OTTHUMP00000206667; Y protein

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Gene Aliases: 2310001E10Rik; 3526402H21Rik; AV083133; Creb; Creb-1; Creb1

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UniProt ID: (Mouse) Q01147, (Rat) P15337

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Entrez Gene ID: (Mouse) 12912, (Rat) 81646

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding enzyme binding histone acetyltransferase binding cAMP response element binding sequence-specific DNA binding transcription regulatory region DNA binding
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein phosphorylation transforming growth factor beta receptor signaling pathway axonogenesis lactation memory circadian rhythm regulation of cell size response to organic substance negative regulation of transcription by competitive promoter binding pituitary gland development cell differentiation mammary gland development positive regulation of transforming growth factor beta3 production secretory granule organization response to glucagon positive regulation of multicellular organism growth response to drug regulation of circadian rhythm regulation of apoptotic process positive regulation of fat cell differentiation positive regulation of osteoclast differentiation positive regulation of transcription, DNA-templated positive regulation of RNA polymerase II transcriptional preinitiation complex assembly positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone secretion positive regulation of lipid biosynthetic process rhythmic process protein stabilization lung epithelium development lung saccule development Type I pneumocyte differentiation cellular response to zinc ion cellular response to growth factor stimulus
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