Invitrogen

JAK3 Monoclonal Antibody (5H2)

2 Published Figures
2 References
View all (38) JAK3 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15561

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200-1:1,000
View 1 publication 1 publication

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5H2

Immunogen

Purified recombinant fragment of human JAK3 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10980143

Product Specific Information

MA5-15561 targets JAK3 in indirect ELISA, FACS, IF, and WB applications and shows reactivity with Human and mouse samples.

The MA5-15561 immunogen is purified recombinant fragment of human JAK3 expressed in E. Coli.

MA5-15561 detects JAK3 which has a predicted molecular weight of approximately 125kDa.

Target Information

Janus kinase 3 (JAK3) phosphorylates members of the STAT (signal transduction and activators of transcription) family. JAK3 is predominantly expressed in immune cells and transduces a signal in response to activation of interleukin receptors. In response to a varitety of cytokine or related factors (e.g., interferon, interleukins), JAKs are activated via phosphorylation at 2 adjacent tyrosine residues. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. However, active JAK1 may be required for complex responses. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Breast-JAK3; JAK-3; JAK3B; Janus kinase 3; Janus kinase 3 (a protein tyrosine kinase, leukocyte); L JAK; L-JAK; Leukocyte janus kinase; Tyrosine-protein kinase JAK3

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Gene Aliases: fae; JAK-3; JAK3; JAK3_HUMAN; JAKL; L-JAK; LJAK; wil

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UniProt ID: (Human) P52333, (Mouse) Q62137

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Entrez Gene ID: (Human) 3718, (Mouse) 16453

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Function(s)
protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding protein phosphatase binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups hormone receptor binding
Process(es)
MAPK cascade adaptive immune response negative regulation of dendritic cell cytokine production protein phosphorylation enzyme linked receptor protein signaling pathway positive regulation of cytosolic calcium ion concentration tyrosine phosphorylation of STAT protein STAT protein import into nucleus cell migration peptidyl-tyrosine phosphorylation B cell differentiation negative regulation of interleukin-10 production negative regulation of interleukin-12 production intracellular signal transduction interleukin-4-mediated signaling pathway peptidyl-tyrosine autophosphorylation positive regulation of T cell proliferation positive regulation of activated T cell proliferation tyrosine phosphorylation of Stat5 protein T cell homeostasis positive regulation of GTPase activity innate immune response negative regulation of FasL biosynthetic process negative regulation of T-helper 1 cell differentiation positive regulation of immune response negative regulation of T cell activation positive regulation of calcium ion transport JAK-STAT cascade involved in growth hormone signaling pathway regulation of T cell apoptotic process negative regulation of thymocyte apoptotic process response to interleukin-2 response to interleukin-4 response to interleukin-15 response to interleukin-9 immune system process transmembrane receptor protein tyrosine kinase signaling pathway phosphorylation cytokine-mediated signaling pathway erythrocyte differentiation positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation lymph node development positive regulation of dendritic cell apoptotic process positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
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