Invitrogen

JAK2 Monoclonal Antibody (691R5)

18 Published Figures
16 References
View all (74) JAK2 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

AHO1352

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
View 9 publications 9 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Immunoprecipitation (IP)

-
View 3 publications 3 publications

Miscellaneous PubMed (Misc)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

691R5

Immunogen

Recombinant fragment of human JAK2 expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536334

Target Information

Janus kinase 2 (JAK2) is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways and is mutated in polycythemia vera. This kinase contains the catalytic domain (JH1) of JAK2. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex. Mutations affecting JAK2 can cause Budd-Chiari syndrome, Polycythemia vera, Thrombocythemia 3, myelofibrosis, or Acute myelogenous leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: EC 2.7.10.2; JAK 2; JAK-2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK 10; kinase Jak2; Tyrosine-protein kinase JAK2

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Gene Aliases: Fd17; JAK2; JTK10; THCYT3

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UniProt ID: (Human) O60674, (Rat) Q62689, (Mouse) Q62120

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Entrez Gene ID: (Human) 3717, (Rat) 24514, (Mouse) 16452

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Function(s)
protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity receptor binding growth hormone receptor binding interleukin-12 receptor binding protein binding ATP binding protein C-terminus binding protein kinase binding heme binding type 1 angiotensin receptor binding acetylcholine receptor binding histone kinase activity (H3-Y41 specific) SH2 domain binding histone binding phosphatidylinositol 3-kinase binding insulin receptor substrate binding metal ion binding peptide hormone receptor binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
activation of MAPKK activity activation of cysteine-type endopeptidase activity involved in apoptotic process response to oxidative stress signal transduction enzyme linked receptor protein signaling pathway G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration JAK-STAT cascade tyrosine phosphorylation of STAT protein STAT protein import into nucleus positive regulation of cell proliferation negative regulation of cell proliferation intrinsic apoptotic signaling pathway in response to oxidative stress hormone-mediated signaling pathway negative regulation of cardiac muscle cell apoptotic process positive regulation of cell-substrate adhesion positive regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway negative regulation of cell-cell adhesion myeloid cell differentiation cell differentiation erythrocyte differentiation positive regulation of cell migration axon regeneration mineralocorticoid receptor signaling pathway positive regulation of insulin secretion response to lipopolysaccharide positive regulation of phosphoprotein phosphatase activity positive regulation of interleukin-1 beta production positive regulation of tumor necrosis factor production positive regulation of protein import into nucleus, translocation response to hydroperoxide tumor necrosis factor-mediated signaling pathway host programmed cell death induced by symbiont response to tumor necrosis factor histone H3-Y41 phosphorylation intracellular signal transduction interleukin-12-mediated signaling pathway peptidyl-tyrosine autophosphorylation tyrosine phosphorylation of Stat3 protein tyrosine phosphorylation of Stat5 protein tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat5 protein activation of JAK2 kinase activity positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of DNA binding negative regulation of DNA binding negative regulation of neuron apoptotic process positive regulation of nitric oxide biosynthetic process positive regulation of cell differentiation negative regulation of heart contraction positive regulation of transcription from RNA polymerase II promoter response to antibiotic protein autophosphorylation platelet-derived growth factor receptor signaling pathway regulation of inflammatory response positive regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation positive regulation of cell activation positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process interferon-gamma-mediated signaling pathway growth hormone receptor signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway positive regulation of growth hormone receptor signaling pathway mammary gland epithelium development response to interleukin-12 cellular response to lipopolysaccharide cellular response to dexamethasone stimulus extrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway positive regulation of apoptotic signaling pathway MAPK cascade adaptive immune response protein phosphorylation apoptotic process movement of cell or subcellular component mesoderm development blood coagulation cell migration actin filament polymerization regulation of cell proliferation regulation of apoptotic process positive regulation of GTPase activity innate immune response regulation of interferon-gamma-mediated signaling pathway immune system process transmembrane receptor protein tyrosine kinase signaling pathway phosphorylation chromatin modification regulation of cell adhesion response to granulocyte macrophage colony-stimulating factor
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