Invitrogen

Fyn Monoclonal Antibody (1S)

Fyn Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Fyn Antibody (AHO0482) in IHC (P)

Immunohistochemical analysis of Fyn in formalin-fixed, paraffin-embedded human lymphoma using a Fyn monoclonal antibody (Product # AHO0482). Detection was performed with a peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

Fyn Antibody in Immunoprecipitation (IP)

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Product Details

AHO0482

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Immunoprecipitation (IP)

8-15 µg

View 3 publications 3 publications

Product Specifications

Published species

Dog, Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

Immunogen

Recombinant protein corresponding to amino acid residues 1-206 of murine p59fynprotein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536311

Product Specific Information

This antibody recognizes epitope aa 26-75.

Staining of formalin-fixed paraffin embedded tissue sections requires boiling the sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Target Information

FYN is a SRC-related kinase expressed in brain, skeletal muscle and adipose tissues. Fyn functions in a large number of pathways and has been shown to play a role in memory. FYN is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri.

Journal of immunology research

Vernot JP,Perdomo-Arciniegas AM,Pérez-Quintero LA,Martínez DF

AHO0482 was used in western blot to utilize a chimeric peptide comprising two functionally different motifs of tip from herpesvirus saimiri which modulates p56Lck in T-Cells

Mon Aug 15 00:00:00 EDT 2016

Interaction of myelin basic protein with cytoskeletal and signaling proteins in cultured primary oligodendrocytes and N19 oligodendroglial cells.

BMC research notes

Boggs JM,Homchaudhuri L,Ranagaraj G,Liu Y,Smith GS,Harauz G

MA1-12607 was used in western blot to study the localization of myelin basic protein isoforms in the central nervous system

Tue Jun 24 00:00:00 EDT 2014

Epidermal hyperplasia and papillomatosis in mice with a keratinocyte-restricted deletion of csk.

Carcinogenesis

Honda K,Sakaguchi T,Sakai K,Schmedt C,Ramirez A,Jorcano JL,Tarakhovsky A,Kamisoyama H,Sakai T

AHO0482 was used in western blot to study the role of csk in epidermal hyperplasia and papillomatosis

Mon Oct 01 00:00:00 EDT 2007

In vitro modeling of human pancreatic duct epithelial cell transformation defines gene expression changes induced by K-ras oncogenic activation in pancreatic carcinogenesis.

Cancer research

Qian J,Niu J,Li M,Chiao PJ,Tsao MS

MA1-12607 was used in immunohistochemistry to investigate the role of K-ras oncogenic activation in pancreatic carcinogenesis

Wed Jun 15 00:00:00 EDT 2005

Possible mechanisms underlying the protective effects of SY-21, an extract of a traditional Chinese herb, on transient brain ischemia/reperfusion-induced neuronal death in rat hippocampus.

Brain research

Chen M,Wang Y,Liu Y,Hou XY,Zhang QG,Meng FJ,Zhang GY

MA1-12607 was used in western blot to study the mechanism underlying the protective effects on transient brain ischemia/reperfusion-induced neuronal death of a traditional Chinese herb

Fri Nov 07 00:00:00 EST 2003

Increased tyrosine phosphorylation of alpha(1C) subunits of L-type voltage-gated calcium channels and interactions among Src/Fyn, PSD-95 and alpha(1C) in rat hippocampus after transient brain ischemia.

Brain research

Hou XY,Zhang GY,Yan JZ,Liu Y

MA1-12607 was used in immunoprecipitation to study phosphorylation of alpha(1C) subunits of L-type voltage-gated calcium channels in rat hippocampus after transient brain ischemia

Fri Jul 25 00:00:00 EDT 2003

Tyrosine kinase and tyrosine phosphatase participate in regulation of interactions of NMDA receptor subunit 2A with Src and Fyn mediated by PSD-95 after transient brain ischemia.

Neuroscience letters

Chen M,Hou X,Zhang G

MA1-12607 was used in western blot to study the role of tyrosine kinase and tyrosine phoshatase on the PSD-95-mediated interaction of the NMDA receptor subunit 2A following transient brain ischemia

Thu Mar 13 00:00:00 EST 2003

Suppression of postsynaptic density protein 95 expression attenuates increased tyrosine phosphorylation of NR2A subunits of N-methyl-D-aspartate receptors and interactions of Src and Fyn with NR2A after transient brain ischemia in rat hippocampus.

Neuroscience letters

Hou XY,Zhang GY,Zong YY

MA1-12607 was used in immunoprecipitation and western blot to study the role of PSD-95 in facilitating NR2A tyrosine phosphorylation by Src family kinases in postischemic brain

Thu Jun 05 00:00:00 EDT 2003

Activation of NMDA receptors and L-type voltage-gated calcium channels mediates enhanced formation of Fyn-PSD95-NR2A complex after transient brain ischemia.

Brain research

Hou XY,Zhang GY,Yan JZ,Chen M,Liu Y

MA1-12607 was used in immunoprecipitation and western blot to study the mechanism of brain ischemia-induced enhancement of NMDA receptor function

Fri Nov 15 00:00:00 EST 2002

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Fyn Monoclonal Antibody (1S)

Fyn Antibody (AHO0482) in IHC (P)

Product Details

AHO0482

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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