Invitrogen

EGF Monoclonal Antibody (3A8)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

M806

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Immunocytochemistry (ICC/IF)

1:50
-

ELISA (ELISA)

0.125-0.25 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3A8

Immunogen

Recombinant Human EGF

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536657

Product Specific Information

The M806 anti-EGF antibody (Clone 3A8) has successfully been paired as the detection antibody in a sandwich ELISA with coating antibody M805 (Clone 1H11).

Typical dilutions for sandwich ELISA include 1-3 µg/mL for coating and 0.125-0.5 µg/mL for detection.

Target Information

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta-urogastrone; EGF; H-EGF; Pro-epidermal growth factor

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Gene Aliases: EGF; HOMG4; URG

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UniProt ID: (Human) P01133

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Entrez Gene ID: (Human) 1950

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Function(s)
protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding calcium ion binding protein binding growth factor activity Wnt-protein binding transmembrane receptor protein tyrosine kinase activator activity Wnt-activated receptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity transmembrane signal receptor
Process(es)
MAPK cascade activation of MAPKK activity activation of MAPK activity angiogenesis platelet degranulation DNA replication signal transduction activation of transmembrane receptor protein tyrosine kinase activity epidermal growth factor receptor signaling pathway STAT protein import into nucleus positive regulation of cell proliferation positive regulation of peptidyl-threonine phosphorylation regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation positive regulation of cerebellar granule cell precursor proliferation positive regulation of catenin import into nucleus ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway positive regulation of phosphorylation positive regulation of DNA binding positive regulation of MAP kinase activity positive regulation of GTPase activity Wnt signaling pathway involved in dorsal/ventral axis specification positive regulation of epidermal growth factor-activated receptor activity positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling branching morphogenesis of an epithelial tube negative regulation of secretion canonical Wnt signaling pathway mammary gland alveolus development ERK1 and ERK2 cascade regulation of calcium ion import negative regulation of cholesterol efflux positive regulation of hyaluronan biosynthetic process regulation of protein localization to cell surface positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of cell motility
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