Invitrogen

beta Actin Loading Control Monoclonal Antibody (BA3R), Biotin

beta Actin Loading Control Antibody in Western Blot (WB)

FIGURE: 1 / 14

beta Actin Loading Control Antibody (MA5-15739-BTIN) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), MDA-MB-231 (Lane 5), PC-12 (Lane 6), RSC96 (Lane 7), tissue extracts of Mouse Lung (Lane 8) and Rat stomach (Lane 9). The blot was probed with beta Actin Loading Control Monoclonal Antibody (BA3R), Biotin (Product # MA5-15739-BTIN, 1 µg/mL) and detected by chemiluminesce... View More

beta Actin Loading Control Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

MA5-15739-BTIN

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 9 publications 9 publications

Flow Cytometry (Flow)

2 µg/test

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

BA3R

Immunogen

Beta-actin N-terminal peptide.

View immunogen

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537661

Product Specific Information

MA5-15739-BTIN has been successfully used for Western blot and FACS applications.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CLEC14A deficiency exacerbates neuronal loss by increasing blood-brain barrier permeability and inflammation.

Journal of neuroinflammation

Kim Y,Lee S,Zhang H,Lee S,Kim H,Kim Y,Won MH,Kim YM,Kwon YG

Published figure using beta Actin Loading Control monoclonal antibody (Product # MA5-15739-BTIN) in Western Blot

Tue Feb 04 00:00:00 EST 2020

Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD.

Frontiers in pharmacology

Grandi A,Zini I,Palese S,Giorgio C,Tognolini M,Marchesani F,Bruno S,Flammini L,Cantoni AM,Castelli R,Lodola A,Fusari A,Barocelli E,Bertoni S

MA5-15739-BTIN was used in Western Blotting to point to a potential role of the EphB/ephrin-B system as a pharmacological target in intestinal inflammatory disorders.

Wed Sep 30 00:00:00 EDT 2020

Estradiol signaling mediates gender difference in visceral adiposity via autophagy.

Cell death & disease

Tao Z,Zheng LD,Smith C,Luo J,Robinson A,Almeida FA,Wang Z,Olumi AF,Liu D,Cheng Z

Published figure using beta Actin Loading Control monoclonal antibody (Product # MA5-15739-BTIN) in Western Blot

Thu Feb 22 00:00:00 EST 2018

NF-κB and IRF1 Induce Endogenous Retrovirus K Expression via Interferon-Stimulated Response Elements in Its 5' Long Terminal Repeat.

Journal of virology

Manghera M,Ferguson-Parry J,Lin R,Douville RN

Published figure using beta Actin Loading Control monoclonal antibody (Product # MA5-15739-BTIN) in Western Blot

Sat Oct 15 00:00:00 EDT 2016

Novel biomarkers of resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus.

Oncotarget

Hastie E,Cataldi M,Moerdyk-Schauwecker MJ,Felt SA,Steuerwald N,Grdzelishvili VZ

Published figure using beta Actin Loading Control monoclonal antibody (Product # MA5-15739-BTIN) in Western Blot

Tue Sep 20 00:00:00 EDT 2016

A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs.

Nature biotechnology

Choi J,Lee S,Mallard W,Clement K,Tagliazucchi GM,Lim H,Choi IY,Ferrari F,Tsankov AM,Pop R,Lee G,Rinn JL,Meissner A,Park PJ,Hochedlinger K

Published figure using beta Actin Loading Control monoclonal antibody (Product # MA5-15739-BTIN) in Western Blot

Sun Nov 01 00:00:00 EDT 2015

Amylin deposition in the brain: A second amyloid in Alzheimer disease?

Annals of neurology

Jackson K,Barisone GA,Diaz E,Jin LW,DeCarli C,Despa F

MA5-15739-BTIN was used in western blot to study the formation of amylin amyloid deposits in the cerebrovascular system and the implications for Alzheimer's disease

Tue Oct 01 00:00:00 EDT 2013

Impairment of Atg5-dependent autophagic flux promotes paraquat- and MPP⁺-induced apoptosis but not rotenone or 6-hydroxydopamine toxicity.

Toxicological sciences : an official journal of the Society of Toxicology

Garcia-Garcia A,Anandhan A,Burns M,Chen H,Zhou Y,Franco R

Published figure using beta Actin Loading Control monoclonal antibody (Product # MA5-15739-BTIN) in Western Blot

Fri Nov 01 00:00:00 EDT 2013

Effects of adult exposure to bisphenol a on genes involved in the physiopathology of rat prefrontal cortex.

PloS one

Castro B,Sánchez P,Torres JM,Ortega E

MA5-15739-BTIN was used in western blot to study the molecular basis for the pathophysiological effects of bisphenol A on the prefrontal cortex of adult rats

Mon May 26 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: actin, beta; Actin, cytoplasmic 1; Bact; actin; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1

View more View less

Gene Aliases: ACT-5; ACTB; actin; Actx; Bact; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1

View more View less

UniProt ID: (Rabbit) P29751, (Human) P60709, (Chicken) P60706, (Dog) O18840, (Rat) P60711, (Mouse) P60710

View more View less

Entrez Gene ID: (Rabbit) 100009272, (Human) 60, (Chicken) 396526, (Dog) 403580, (Rat) 81822, (Mouse) 11461

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton protein binding ATP binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding protein kinase binding structural constituent of postsynaptic actin cytoskeleton actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Process(es)

retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation axonogenesis cell motility postsynaptic actin cytoskeleton organization retina development in camera-type eye cellular response to electrical stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

beta Actin Loading Control Monoclonal Antibody (BA3R), Biotin

beta Actin Loading Control Antibody (MA5-15739-BTIN) in WB

Product Details

MA5-15739-BTIN

Western Blot (WB)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!