Invitrogen

Goat anti-Rat IgG (H+L) Secondary Antibody, WesternDot™ 800

Rat IgG (H+L) Secondary Antibody in Western Blot (WB)

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Rat IgG (H+L) Secondary Antibody (W10821) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of NCCIT (Lane 1) and F9 (Lane 2). The blots were probed with Anti-alpha Tubulin Rat Monoclonal Antibody (Product # MA1-80017, 1 µg/ml) and detected by chemiluminescence using Goat anti-Rat IgG Secondary Antibody, WesternDot® 800 (Product # W10821) at dilutions 1:2,000 (Fig. 1) and 1:5,000 (Fig. 2). A 52 kDa band corresponding to alph... View More

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Product Details

W10821

Applications

Tested Dilution

Western Blot (WB)

1:2,000-1:5,000

Product Specifications

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Rat Immunoglobin

Conjugate

WesternDot™ 800 WesternDot™ 800 WesternDot™ 800

Form

Liquid

Storage conditions

4° C, do not freeze

RRID

AB_2556803

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

WesternDot fluorescently labeled secondary antibodies deliver superior signal-to-noise ratios and exhibit comparable sensitivity to enhanced chemiluminescence (ECL)-based methods in western blot applications. No stripping and re-probing or multiple blots are required for multiple protein detection as commonly needed with ECL or colorimetric detection. Differently labeled WesternDot antibodies can be applied to a single blot for simultaneous detection of multiple proteins using standard gel or blot imaging platforms (a list of instruments that are compatible with WesternDot detection can be found in the user manual below). Our WesternDot antibodies utilize Qdot nanocrystals enhanced with VIVID technology, making them brighter than the original Qdot reagents. Additionally, they have reduced intensity differences between the different colors, simplifying multiplex applications.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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