Invitrogen

HisG Epitope Tag Monoclonal Antibody

HisG Epitope Tag Antibody in Immunocytochemistry (ICC/IF)

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HisG Epitope Tag Antibody (R940-25) in ICC/IF

Immunofluorescent analysis of HisG was done on HEK-293 cells transiently overexpressing HisG-RPS6 and untransfected cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with HisG Mouse Monoclonal Antibody (Product # R940-25) at 1:250 dilution in 1% B... View More

HisG Epitope Tag Antibody in Western Blot (WB)

HisG Epitope Tag Antibody in Immunoprecipitation (IP)

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Product Details

R940-25

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

1:250

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

In vitro Assay (IV)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Immunogen

6x His synthetic peptide: -His-His-His-His-His-His-Gly

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

PBS

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2556557

Product Specific Information

NOTE: R940-25 was previously sold under product #46-1008. When re-ordering, please use #R940-25.

This antibody is designed to specifically detect recombinant proteins containing the seven amino acid 6xHis-Gly epitope. This epitope is present in many expression vectors encoding an N-terminal 6xHis tag. The HisG antibody recognizes the following sequence: -His-His-His-His-His-His-Gly.

This product contains enough material for 25 Western blots. It has been tested against purified Positope™ control protein (100 ng). The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5. For Western blot, dilute in PBS containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM).

Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.

This antibody has also been used successfully to immunoprecipitate fusion proteins that contain the 6xHis tag.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. HisG antibodies allow detection of recombinant proteins containing a polyhistidine sequence: His-His-His-His-His-His-Gly (6xHis-Gly epitope). HisG antibodies can be used to detect expression of fusion proteins from bacterial, insect, and mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis.

Nature communications

Bieluszewski T,Sura W,Dziegielewski W,Bieluszewska A,Lachance C,Kabza M,Szymanska-Lejman M,Abram M,Wlodzimierz P,De Winne N,De Jaeger G,Sadowski J,Côté J,Ziolkowski PA

R940-25 was used in Western Blot to describe Arabidopsis homologs of the NuA4 scaffold proteins Enhancer of Polycomb-Like 1 (AtEPL1) and Esa1-Associated Factor 1 (AtEAF1).

Wed Jan 12 00:00:00 EST 2022

NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis.

Nature communications

Bieluszewski T,Sura W,Dziegielewski W,Bieluszewska A,Lachance C,Kabza M,Szymanska-Lejman M,Abram M,Wlodzimierz P,De Winne N,De Jaeger G,Sadowski J,Côté J,Ziolkowski PA

R940-25 was used in Western Blot to describe Arabidopsis homologs of the NuA4 scaffold proteins Enhancer of Polycomb-Like 1 (AtEPL1) and Esa1-Associated Factor 1 (AtEAF1).

Wed Jan 12 00:00:00 EST 2022

PKA-binding domain of AKAP8 is essential for direct interaction with DPY30 protein.

The FEBS journal

Bieluszewska A,Weglewska M,Bieluszewski T,Lesniewicz K,Poreba E

R940-25 was used in Western Blotting to characterise the interaction between A kinase-anchoring protein 8 and the core subunit of all known H3K4 histone methyltransferase complexes-DPY30 protein.

Thu Mar 01 00:00:00 EST 2018

Regulation of Receptor for Advanced Glycation End Products (RAGE) Ectodomain Shedding and Its Role in Cell Function.

The Journal of biological chemistry

Braley A,Kwak T,Jules J,Harja E,Landgraf R,Hudson BI

R940-25 was used in western blot to study ectodomain shedding and its role in cell function by regulation of receptor for advanced glycation end products (RAGE)

Fri Jun 03 00:00:00 EDT 2016

Inhibition of mouse SP2/0 myeloma cell growth by the B7-H4 protein vaccine.

BMB reports

Mu N,Liu N,Hao Q,Xu Y,Li J,Li W,Wu S,Zhang C,Su H

R940-25 was used in western blot to assess the anti-tumor effect of the TT-rhB7-H4IgV vaccine

Tue Jul 01 00:00:00 EDT 2014

Canine placental prostaglandin E2 synthase: expression, localization, and biological functions in providing substrates for prepartum PGF2alpha synthesis.

Biology of reproduction

Gram A,Fox B,Büchler U,Boos A,Hoffmann B,Kowalewski MP

R940-25 was used in Western Blotting to suggest that PGE2 could be used locally as a substrate for prepartum PGF2alpha synthesis in the dog.

Mon Dec 01 00:00:00 EST 2014

Biosynthesis and degradation of canine placental prostaglandins: prepartum changes in expression and function of prostaglandin F2α-synthase (PGFS, AKR1C3) and 15-hydroxyprostaglandin dehydrogenase (HPGD).

Biology of reproduction

Gram A,Büchler U,Boos A,Hoffmann B,Kowalewski MP

R940-25 was used in Western Blotting to study the regulation of canine uterine PGF2alpha availability and eventual prepartum release in luteolytic amounts in dogs.

Mon Jul 01 00:00:00 EDT 2013

The balance between two isoforms of LEF-1 regulates colon carcinoma growth.

BMC gastroenterology

Wang SH,Nan KJ,Wang YC,Wang WJ,Tian T

Published figure using HisG Epitope Tag monoclonal antibody (Product # R940-25) in Western Blot

Mon May 28 00:00:00 EDT 2012

TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity.

The Journal of biological chemistry

Chen KR,Chang CH,Huang CY,Lin CY,Lin WY,Lo YC,Yang CY,Hsing EW,Chen LF,Shih SR,Shiau AL,Lei HY,Tan TH,Ling P

R940-25 was used in Western Blotting to demonstrate a critical role for TBK1-associated protein in endolysosomes in linking retinoic acid-inducible gene I-like receptors to antiviral immunity.

Fri Sep 14 00:00:00 EDT 2012

The major antigenic membrane protein of "Candidatus Phytoplasma asteris" selectively interacts with ATP synthase and actin of leafhopper vectors.

PloS one

Galetto L,Bosco D,Balestrini R,Genre A,Fletcher J,Marzachì C

R940-25 was used in western blot to identify vector proteins that interact with Amp and investigate their role in transmission of Candidatus Phytoplasma asteris

Tue Dec 06 00:00:00 EST 2011

High-throughput subcellular protein localization using transfected-cell arrays. Subcellular protein localization using cell arrays.

Methods in molecular biology (Clifton, N.J.)

Hu Y,Janitz M

R940-25 was used in In vitro experiments to facilitate the precise determination of the localizations of numerous recombinant proteins in a single experiment, making it currently the most efficient technique for high-throughput protein co-localization screening.

Tue Mar 22 00:00:00 EDT 2011

DIP (mDia interacting protein) is a key molecule regulating Rho and Rac in a Src-dependent manner.

The EMBO journal

Meng W,Numazaki M,Takeuchi K,Uchibori Y,Ando-Akatsuka Y,Tominaga M,Tominaga T

R940-25 was used in Immunoprecipitation to characterise the molecular interactions and roles of mDia interacting protein in regulating Rho and Rac.

Wed Feb 25 00:00:00 EST 2004

Endothelial induction of fgl2 contributes to thrombosis during acute vascular xenograft rejection.

Journal of immunology (Baltimore, Md. : 1950)

Ghanekar A,Mendicino M,Liu H,He W,Liu M,Zhong R,Phillips MJ,Levy GA,Grant DR

R940-25 was used in western blot to test if induction of fgl2 on the vascular endothelium of xenografts contributes to thrombosis associated with acute vascular rejection

Sat May 01 00:00:00 EDT 2004

A novel single amino acid deletion caspase-8 mutant in cancer cells that lost proapoptotic activity.

The Journal of biological chemistry

Liu B,Peng D,Lu Y,Jin W,Fan Z

R940-25 was used in immunoprecipitation to characterize a novel caspase-8 mutant, Delta Leu62casp-8

Fri Aug 16 00:00:00 EDT 2002

Punctin, a novel ADAMTS-like molecule, ADAMTSL-1, in extracellular matrix.

The Journal of biological chemistry

Hirohata S,Wang LW,Miyagi M,Yan L,Seldin MF,Keene DR,Crabb JW,Apte SS

R940-25 was used in western blot to describe the localization and composition of Punctin (ADAMTSL-1)

Fri Apr 05 00:00:00 EST 2002

Elevated retinoic acid receptor beta(4) protein in human breast tumor cells with nuclear and cytoplasmic localization.

Proceedings of the National Academy of Sciences of the United States of America

Sommer KM,Chen LI,Treuting PM,Smith LT,Swisshelm K

R940-25 was used in immunocytochemistry to describe the expression and structure of human RARbeta(4)

Tue Jul 20 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: 6xHis-Gly; 6xHis-Glycine; His G; His Tag; Histidine Tag

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HisG Epitope Tag Monoclonal Antibody

HisG Epitope Tag Antibody (R940-25) in ICC/IF

Product Details

R940-25

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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