Remel™ Brilliant Green Agar

Selectively isolate Salmonellae, other than Salmonella enterica serovars Typhi and Paratyphi from food samples or other material using Thermo Scientific™ Remel™ Brilliant Green Agar (Dehydrated). Kristensen developed Brilliant Green Agar for isolation of Salmonella¹. Moats added novobiocin to the medium which inhibited growth of Proteus in cultures from beef and poultry products². Brilliant Green Agar is recommended by the American Public Health Association (APHA) for selective isolation of Salmonella from food products³.

This medium contains casein and meat peptones which provide nitrogen, amino acids and peptides necessary for bacterial growth. Yeast extract is a source of B-complex vitamins. Sodium chloride is a source of essential electrolytes and maintains osmotic equilibrium. Lactose- and sucrose-fermenting colonies occasionally growing on this medium will be yellow-green in color surrounded by a yellow-green zone. Salmonella colonies range from reddish or pink to nearly white in color with a red zone. Novobiocin suppresses the growth of Proteus and, to a lesser extent, Escherichia coli, Citrobacter, and Pseudomonas. In separate studies, Devenish and Cooke added novobiocin to Brilliant Green Agar to further improve selectivity^4,5.

• Selective media - Brilliant green dye in the medium makes this medium inhibits the growth of non-target organisms.

Easy-to-read – Phenol red in the medium serves as an acid base indicator giving yellow color to lactose and sucrose fermenting bacteria.

• Corresponds to the medium recommended by the APHA³.