Qubit™ microRNA Assay Kits

Achieve accurate and precise quantification of small RNA (∼20 nucleotides or base pairs) with Qubit miRNA (microRNA) Assay Kits. These miRNA quantification kits enable quick detection of all types of small RNA, including microRNA and siRNA, and both single stranded and double stranded RNA. The assay is highly selective for small RNA over rRNA or large mRNA, and can tolerate contaminants such as salts, solvents, or detergents.

The Qubit miRNA Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of microRNA samples. This assay is highly selective for miRNA over rRNA or large mRNA (>1000 bp). Depending on sample volume, the assay kit is designed to be accurate for initial miRNA sample concentrations of 0.025 to 150 ng/μL, providing a detection range of 0.5−150 ng.

The Qubit microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using the Qubit Fluorometer.

Notes
• Use with thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200-μL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer