For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Recombinant full-length human AMPK (combination of A1/B2/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag.
• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
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LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant
AMPK (A1/B2/G1) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The beta subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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