Invitrogen

Human ACVR2A, GST Tag Recombinant Protein

Product Details

PV6124

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

Full length

Tag

GST-tag

Molecular weight

85.3 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 0.5mM EDTA, 150mM NaCl, 0.02% NP-40, 50% glycerol, 2mM DTT

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This recombinant fusion protein consists of full-length activin A receptor kinase and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
• Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
• Expressed in insect cells
• GST tagged for use with Eu-labeled GST antibody

Easily Screen for Kinase Binding
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

ACVR2A for the LanthaScreen Kinase Binding Assay

This insect cell-expressed recombinant fusion protein of full-length ACVR2A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with these reagents:
• Tracer 236 (Cat No. PV5592)
• Eu-labeled GST Antibody (Cat No. PV5594)

Related Link
• Learn more about the LanthaScreen™ Eu Binding Assay.

Protein Form: Full Length

Target Information

Activin A receptor kinase (ACVR2A) is a type II member of the TGF-beta family of receptor Serine/Threonine kinases. ACVR2A is involved in the activin and myostatin signaling pathway. (ACVR2A Aliases: ACVR2, ACTRII). These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling, and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: activin A receptor type IIA; activin A receptor, type IIA; Activin receptor type IIA; Activin receptor type-2A; ACTR-IIA