Invitrogen

Human CDK8/Cyclin C, His Tag Recombinant Protein

Product Details

PR7261B

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Baculovirus

Amino acid sequence

Full length

Tag

His-tag

Molecular weight

97 kDa

Class

Recombinant

Type

Protein

Purity

80% by SDS-PAGE

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris, pH 7.5, with 2mM DTT, 150mM NaCl, 0.5mM EDTA, 50% glycerol, 0.02% Triton X-100

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Protein Form: Full Length

Target Information

CDK8 phosphorylates the carboxy-terminal domain of the largest subunit of RNA polymerase II. This protein is coexpressed and copurified with Cyclin C. CDK8 is part of a family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34/Cdc 2, and a related protein Cdk 2, function late in the cycle while Cdk 4 and Cdk 6 are critically involved in G1 to S progression. Because of analogy with yeast Cdk 8, it has been proposed that the human Cdk 8 Cyclin C may interact with RNA polymerase, thus influencing the transcription mechanism(s) that lead to cell division.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDK8 protein kinase; Cell division protein kinase 8; Cyclin-dependent kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; MGC126074; MGC126075; Protein kinase K35