Invitrogen

Phospho-IRF3 (Ser385) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

6 References

View all (71) IRF3 antibodies

Datasheet

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Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-IRF3 (Ser385) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

Phospho-IRF3 (Ser385) Antibody (PA5-36775) in IHC (P)

Immunohistochemistry analysis of Phospho-IRF3 (Ser385) in paraffin-embedded human colon carcinoma and breast carcinoma tissue. Samples were incubated with Phospho-IRF3 (Ser385) polyclonal antibody (Product # PA5-36775).

Phospho-IRF3 (Ser385) Antibody in Western Blot (WB)

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Product Details

PA5-36775

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human IRF-3 around the phosphorylation site of Serine 385

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2553722

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 47 and 55 kDa.

Purity is >95% by SDS-PAGE.

Target Information

IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Estrogen receptor alpha signaling in dendritic cells modulates autoimmune disease phenotype in mice.

EMBO reports

Khaw YM,Anwar S,Zhou J,Kawano T,Lin PC,Otero A,Barakat R,Drnevich J,Takahashi T,Ko CJ,Inoue M

PA5-36775 was used in Western Blotting to demonstrate that ERα deletion in dendritic cells (DCs) of mice induces severe neurodegeneration in the central nervous system in a mouse EAE model and resistance to interferon beta (IFNβ), a first-line MS treatment.

Mon Mar 06 00:00:00 EST 2023

Estrogen receptor alpha signaling in dendritic cells modulates autoimmune disease phenotype in mice.

EMBO reports

Khaw YM,Anwar S,Zhou J,Kawano T,Lin PC,Otero A,Barakat R,Drnevich J,Takahashi T,Ko CJ,Inoue M

Mon Mar 06 00:00:00 EST 2023

Keratinocytes sense and eliminate CRISPR DNA through STING/IFN-κ activation and APOBEC3G induction.

The Journal of clinical investigation

Sarkar MK,Uppala R,Zeng C,Billi AC,Tsoi LC,Kidder A,Xing X,Perez White BE,Shao S,Plazyo O,Sirobhushanam S,Xing E,Jiang Y,Gallagher KA,Voorhees JJ,Kahlenberg JM,Gudjonsson JE

Published figure using Phospho-IRF3 (Ser385) polyclonal antibody (Product # PA5-36775) in Western Blot

Mon May 01 00:00:00 EDT 2023

Loss of Zfp335 triggers cGAS/STING-dependent apoptosis of post-β selection thymocytes.

Nature communications

Ratiu JJ,Barclay WE,Lin E,Wang Q,Wellford S,Mehta N,Harnois MJ,DiPalma D,Roy S,Contreras AV,Shinohara ML,Wiest D,Zhuang Y

PA5-36775 was used in Proximity Ligation Assay (PLA) to identify Zfp335 as a key transcription factor regulating the survival of proliferating post-β-selection thymocytes and demonstrates a key role for the cGAS/STING pathway in driving apoptosis of developing T cells.

Thu Oct 06 00:00:00 EDT 2022

G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells.

Nucleic acids research

Miglietta G,Russo M,Duardo RC,Capranico G

PA5-36775 was used in Western Blot to identify two G-quadruplex binders as regulators of signaling pathways in cancer cells.

Fri Jul 09 00:00:00 EDT 2021

Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity.

Nature communications

von Roemeling CA,Wang Y,Qie Y,Yuan H,Zhao H,Liu X,Yang Z,Yang M,Deng W,Bruno KA,Chan CK,Lee AS,Rosenfeld SS,Yun K,Johnson AJ,Mitchell DA,Jiang W,Kim BYS

PA5-36775 was used in Western Blotting to show stimulation of glioma cell phagocytosis by blockage of anti-phagocytosis molecule CD47 is an ineffective process.

Fri Mar 20 00:00:00 EDT 2020

Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity.

Nature communications

von Roemeling CA,Wang Y,Qie Y,Yuan H,Zhao H,Liu X,Yang Z,Yang M,Deng W,Bruno KA,Chan CK,Lee AS,Rosenfeld SS,Yun K,Johnson AJ,Mitchell DA,Jiang W,Kim BYS

PA5-36775 was used in Western Blotting to show stimulation of glioma cell phagocytosis by blockage of anti-phagocytosis molecule CD47 is an ineffective process.

Fri Mar 20 00:00:00 EDT 2020

(+)-Naloxone blocks Toll-like receptor 4 to ameliorate deleterious effects of stress on male mouse behaviors.

Brain, behavior, and immunity

Medina-Rodriguez EM,Rice KC,Beurel E,Jope RS

PA5-36775 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that blocking TLR4 with (+)-naloxone effectively diminishes several detrimental responses to stress and raise the possibility that (+)-naloxone may be a feasible intervention for depression.

Sun Nov 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Interferon regulatory factor 3; IRF-3; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3

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Gene Aliases: C920001K05Rik; IIAE7; IRF-3; IRF3

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UniProt ID: (Human) Q14653, (Mouse) P70671

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Entrez Gene ID: (Human) 3661, (Mouse) 54131

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription cofactor activity protein binding identical protein binding protein homodimerization activity regulatory region DNA binding transcription factor activity, sequence-specific DNA binding transcription regulatory region DNA binding winged helix/forkhead transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter apoptotic process cellular response to DNA damage stimulus lipopolysaccharide-mediated signaling pathway negative regulation of type I interferon production positive regulation of type I interferon production positive regulation of interferon-alpha production positive regulation of interferon-beta production TRIF-dependent toll-like receptor signaling pathway MDA-5 signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling response to exogenous dsRNA type I interferon biosynthetic process positive regulation of cytokine secretion defense response to virus interferon-gamma-mediated signaling pathway type I interferon signaling pathway positive regulation of type I interferon-mediated signaling pathway cellular response to dsRNA macrophage apoptotic process programmed necrotic cell death immune system process transcription, DNA-templated regulation of transcription, DNA-templated response to bacterium response to lipopolysaccharide innate immune response negative regulation of interferon-beta biosynthetic process negative regulation of defense response to virus by host cellular response to lipopolysaccharide

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-IRF3 (Ser385) Polyclonal Antibody

Phospho-IRF3 (Ser385) Antibody (PA5-36775) in IHC (P)

Product Details

PA5-36775

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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