Invitrogen

PD-1 Polyclonal Antibody

PD-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PD-1 Antibody (PA5-20350) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human brain tissue using PD-1 Polyclonal Antibody (Product # PA5-20350) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. ... View More

PD-1 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-20350

Applications

Tested Dilution

Publications

Western Blot (WB)

1-4 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

2.5-5 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 16 amino acid peptide from near the carboxy terminus of human PD-1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153220

Product Specific Information

Despite its predicted molecular weight, PD-1 often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is THP-1 cell lysate.

PA5-20350 can be used with blocking peptide PEP-0470.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

PA5-20350 was used in Immunohistochemistry to establish both direct and indirect roles for ILC2 in peri-aortic CaPO4 injury-induced mouse abdominal aortic aneurysms.

Wed Mar 01 00:00:00 EST 2023

Human papillomavirus infection can alter the level of tumour stemness and T cell infiltration in patients with head and neck squamous cell carcinoma.

Frontiers in immunology

Meng L,Lu H,Li Y,Zhao J,He S,Wang Z,Shen J,Huang H,Xiao J,Sooranna SR,Song J

PA5-20350 was used in Immunohistochemistry to suggest that the stemness features of tumour cells are not only associated with the prognostic risk, but that it could also affect the immune cell interactions and associated signalling pathways.

Fri Nov 25 00:00:00 EST 2022

In PD-1+ human colon cancer cells NIVOLUMAB promotes survival and could protect tumor cells from conventional therapies.

Journal for immunotherapy of cancer

Ieranò C,Righelli D,D'Alterio C,Napolitano M,Portella L,Rea G,Auletta F,Santagata S,Trotta AM,Guardascione G,Liotti F,Prevete N,Maiolino P,Luciano A,Barbieri A,Di Mauro A,Roma C,Esposito Abate R,Tatangelo F,Pacelli R,Normanno N,Melillo RM,Scala S

PA5-20350 was used in Immunohistochemistry to show that in PD-1+ human colon cancer cells, NIVO activates tumor survival pathways and could protect tumor cells from conventional therapies.

Tue Mar 01 00:00:00 EST 2022

Molecular mechanism of SHP2 activation by PD-1 stimulation.

Science advances

Marasco M,Berteotti A,Weyershaeuser J,Thorausch N,Sikorska J,Krausze J,Brandt HJ,Kirkpatrick J,Rios P,Schamel WW,Köhn M,Carlomagno T

PA5-20350 was used in Western Blot to explain the structural basis and provide functional evidence for the mechanism of PD-1-mediated SHP2 activation. The study demonstartes that full activation is obtained only upon phosphorylation of both ITIM and ITSM: ITSM binds C-SH2 with strong affinity, recruiting SHP2 to PD-1, while ITIM binds N-SH2, displacing it from the catalytic pocket and activating SHP2.

Wed Jan 01 00:00:00 EST 2020

Targeting CXCR4 potentiates anti-PD-1 efficacy modifying the tumor microenvironment and inhibiting neoplastic PD-1.

Journal of experimental & clinical cancer research : CR

D'Alterio C,Buoncervello M,Ieranò C,Napolitano M,Portella L,Rea G,Barbieri A,Luciano A,Scognamiglio G,Tatangelo F,Anniciello AM,Monaco M,Cavalcanti E,Maiolino P,Romagnoli G,Arra C,Botti G,Gabriele L,Scala S

PA5-20350 was used in Immunohistochemistry to evaluate the TME.

Mon Oct 28 00:00:00 EDT 2019

Targeting CXCR4 potentiates anti-PD-1 efficacy modifying the tumor microenvironment and inhibiting neoplastic PD-1.

Journal of experimental & clinical cancer research : CR

PA5-20350 was used in Immunohistochemistry to evaluate the TME.

Mon Oct 28 00:00:00 EDT 2019

Targeting CXCR4 potentiates anti-PD-1 efficacy modifying the tumor microenvironment and inhibiting neoplastic PD-1.

Journal of experimental & clinical cancer research : CR

PA5-20350 was used in Immunohistochemistry to evaluate the TME.

Mon Oct 28 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: CD279; hPD1; mPD-1; programmed cell death 1 protein; Programmed cell death protein 1; programmed cell death protein 1-like; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2

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Gene Aliases: CD279; hPD-1; hPD-l; hSLE1; Ly101; PD-1; PD1; Pdc1; PDCD1; SLEB2

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UniProt ID: (Human) Q15116, (Mouse) Q02242

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Entrez Gene ID: (Human) 5133, (Rat) 301626, (Mouse) 18566

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Function(s)

signal transducer activity protein binding molecular_function

Process(es)

negative regulation of tolerance induction apoptotic process humoral immune response signal transduction multicellular organism development T cell costimulation negative regulation of apoptotic process positive regulation of T cell apoptotic process biological_process positive regulation of apoptotic process immune system process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PD-1 Polyclonal Antibody

PD-1 Antibody (PA5-20350) in IHC (P)

Product Details

PA5-20350

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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