Invitrogen

HDAC1 Polyclonal Antibody, Alexa Fluor™ 555

HDAC1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 1

HDAC1 Antibody (PA1-860-A555) in ICC/IF

Immunofluorescent analysis of HDAC1 (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a HDAC1 Polyclonal Antibody, AlexaFluor 555 conjugate (Product # PA1-860-A555) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. I... View More

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Product Details

PA1-860-A555

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1-10 µg/mL

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1

View immunogen

Conjugate

Alexa Fluor™ 555 Alexa Fluor™ 555 Alexa Fluor™ 555

Excitation/Emission Max

553/568 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2663463

Product Specific Information

PA1860A555 detects histone deacetylase 1 (HDAC1) from human, mouse, rat, hamster and canine tissues and cells.

The PA1860A555 immunogen is a synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1. This immunizing peptide (Cat. # PEP-069) is available for use in neutralization and control experiments.

Target Information

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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