Invitrogen

GFAP Polyclonal Antibody

GFAP Antibody in Immunohistochemistry (Frozen) (IHC (F))

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GFAP Antibody (OPA1-06100) in IHC (F)

Deep tissue imaging with non-curing, refractive index matched SlowFade™ Glass. Cryo-preserved rat brain sections (100 µm thick), stained for tubulin (red) with Mouse Anti-Beta3-Tubulin (Cat. No. MA1-118) and GFAP (yellow) Rabbit Anti-GFAP (Cat. No. OPA1-06100). Targets were detected with Alexa Fluor™ Plus 594 Goat Anti-Mouse (Cat. No. A-11032) and Alexa Fluor™ Plus 647 Goat Anti-Rabbit (Cat. No. A-32733). N... View More

GFAP Antibody in Immunohistochemistry (IHC)

GFAP Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

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Product Details

OPA1-06100

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human spinal cord.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_325657

Product Specific Information

OPA1-06100 detects glial fibrillary acid protein (GFAP) from human, mouse, zebrafish, and rat tissues.

OPA1-06100 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects a single protein representing GFAP rat brain homogenate. Immunohistochemical staining of GFAP in rat brain with OPA1-6100 results in intense staining of astrocytes and Schwann cells.

The OPA1-06100 immunogen is cytoskeletal preparation of human spinal cord.

Store at 4ºC or in small aliquots at -20ºC.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Insulin Diminishes Superoxide Increase in Cytosol and Mitochondria of Cultured Cortical Neurons Treated with Toxic Glutamate.

International journal of molecular sciences

Pinelis V,Krasilnikova I,Bakaeva Z,Surin A,Boyarkin D,Fisenko A,Krasilnikova O,Pomytkin I

OPA1-06100 was used in Cell Culture to show that MK 801, an inhibitor of NMDAR-gated Ca2+ influx, completely abrogated the glutamate effects in both the presence and absence of insulin.

Thu Oct 20 00:00:00 EDT 2022

Full-length Dhh and N-terminal Shh act as competitive antagonists to regulate angiogenesis and vascular permeability.

Cardiovascular research

Hollier PL,Chapouly C,Diop A,Guimbal S,Cornuault L,Gadeau AP,Renault MA

OPA1-06100 was used in Immunocytochemistry to demonstrate that ectopically administered N-terminal Sonic Hedgehog (N-Shh) and endogenous endothelial-derived Desert Hedgehog (Dhh) induce opposite effects in endothelial cells (ECs).

Mon Nov 01 00:00:00 EDT 2021

Lasting alterations induced in glial cell phenotypes by short exposure to alcohol during embryonic development in zebrafish.

Addiction biology

Chatterjee D,Mahabir S,Chatterjee D,Gerlai R

Published figure using GFAP polyclonal antibody (Product # OPA1-06100) in Immunohistochemistry

Fri Jan 01 00:00:00 EST 2021

Lasting alterations induced in glial cell phenotypes by short exposure to alcohol during embryonic development in zebrafish.

Addiction biology

Chatterjee D,Mahabir S,Chatterjee D,Gerlai R

Published figure using GFAP polyclonal antibody (Product # OPA1-06100) in Immunohistochemistry

Fri Jan 01 00:00:00 EST 2021

The TGFβ/Notch axis facilitates Müller cell-to-epithelial transition to ultimately form a chronic glial scar.

Molecular neurodegeneration

Conedera FM,Pousa AMQ,Mercader N,Tschopp M,Enzmann V

Published figure using GFAP polyclonal antibody (Product # OPA1-06100) in Immunocytochemistry

Thu Sep 30 00:00:00 EDT 2021

The TGFβ/Notch axis facilitates Müller cell-to-epithelial transition to ultimately form a chronic glial scar.

Molecular neurodegeneration

Conedera FM,Pousa AMQ,Mercader N,Tschopp M,Enzmann V

Published figure using GFAP polyclonal antibody (Product # OPA1-06100) in Immunocytochemistry

Thu Sep 30 00:00:00 EDT 2021

The TGFβ/Notch axis facilitates Müller cell-to-epithelial transition to ultimately form a chronic glial scar.

Molecular neurodegeneration

Conedera FM,Pousa AMQ,Mercader N,Tschopp M,Enzmann V

Published figure using GFAP polyclonal antibody (Product # OPA1-06100) in Immunocytochemistry

Thu Sep 30 00:00:00 EDT 2021

The TGFβ/Notch axis facilitates Müller cell-to-epithelial transition to ultimately form a chronic glial scar.

Molecular neurodegeneration

Conedera FM,Pousa AMQ,Mercader N,Tschopp M,Enzmann V

Published figure using GFAP polyclonal antibody (Product # OPA1-06100) in Immunocytochemistry

Thu Sep 30 00:00:00 EDT 2021

Desert Hedgehog-Driven Endothelium Integrity Is Enhanced by Gas1 (Growth Arrest-Specific 1) but Negatively Regulated by Cdon (Cell Adhesion Molecule-Related/Downregulated by Oncogenes).

Arteriosclerosis, thrombosis, and vascular biology

Chapouly C,Hollier PL,Guimbal S,Cornuault L,Gadeau AP,Renault MA

OPA1-06100 was used in Immunocytochemistry to investigate whether hedgehog coreceptors Gas1 (growth arrest-specific 1) and Cdon (cell adhesion molecule-related/downregulated by oncogenes) may be used as therapeutic targets to modulate Dhh signaling in ECs.

Tue Dec 01 00:00:00 EST 2020

Retinal microglia signaling affects Müller cell behavior in the zebrafish following laser injury induction.

Glia

Conedera FM,Pousa AMQ,Mercader N,Tschopp M,Enzmann V

OPA1-06100 was used in Immunohistochemistry-immunofluorescence to illustraate that microglia and Müller cell signaling is pivotal to unlock the regenerative potential of Müller cells in order to repair the damaged retina.

Sat Jun 01 00:00:00 EDT 2019

Targeting Brain Disease in MPSII: Preclinical Evaluation of IDS-Loaded PLGA Nanoparticles.

International journal of molecular sciences

Rigon L,Salvalaio M,Pederzoli F,Legnini E,Duskey JT,D'Avanzo F,De Filippis C,Ruozi B,Marin O,Vandelli MA,Ottonelli I,Scarpa M,Tosi G,Tomanin R

OPA1-06100 was used in Immunohistochemistry to show a significant reduction of GAG deposits in liver and brain tissues, as well as a reduction of some neurological and inflammatory markers (i.e., LAMP2, CD68, GFAP), highlighting a general improvement of the brain pathology.

Wed Apr 24 00:00:00 EDT 2019

A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage.

Scientific reports

Aum DJ,Vellimana AK,Singh I,Milner E,Nelson JW,Han BH,Zipfel GJ

Published figure using GFAP polyclonal antibody (Product # OPA1-06100) in Immunofluorescence

Tue Aug 22 00:00:00 EDT 2017

The nucleotide-binding leucine-rich repeat (NLR) family member NLRX1 mediates protection against experimental autoimmune encephalomyelitis and represses macrophage/microglia-induced inflammation.

The Journal of biological chemistry

Eitas TK,Chou WC,Wen H,Gris D,Robbins GR,Brickey J,Oyama Y,Ting JP

OPA1-06100 was used in western blot to study the mechanism by which NLRX1 protects against experimental autoimmune encephalomyelitis

Fri Feb 14 00:00:00 EST 2014

Miz1 is required to maintain autophagic flux.

Nature communications

Wolf E,Gebhardt A,Kawauchi D,Walz S,von Eyss B,Wagner N,Renninger C,Krohne G,Asan E,Roussel MF,Eilers M

OPA1-06100 was used in immunohistochemistry to study the role of Miz1 in autophagy

Thu May 01 00:00:00 EDT 2014

Behavioral and neurochemical alterations in mice deficient in anaplastic lymphoma kinase suggest therapeutic potential for psychiatric indications.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

Bilsland JG,Wheeldon A,Mead A,Znamenskiy P,Almond S,Waters KA,Thakur M,Beaumont V,Bonnert TP,Heavens R,Whiting P,McAllister G,Munoz-Sanjuan I

OPA1-06100 was used in immunocytochemistry to investigate the role of receptor tyrosine kinase in the hematopoietic and neural systems

Fri Feb 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; zGFAP; intermediate filament protein; zrf-1 antigen

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Gene Aliases: AI836096; ALXDRD; cb345; etID36982.3; GFAP; gfapl; wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485

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UniProt ID: (Human) E9PAX3, (Rat) P47819, (Mouse) P03995, (Zebrafish) Q58EE9

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Entrez Gene ID: (Human) 2670, (Rat) 24387, (Mouse) 14580, (Zebrafish) 30646

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Function(s)

glycoprotein binding integrin binding structural constituent of cytoskeleton protein binding kinase binding structural molecule activity

Process(es)

response to wounding positive regulation of Schwann cell proliferation negative regulation of neuron projection development astrocyte development extracellular matrix organization neuron projection regeneration intermediate filament-based process intermediate filament organization regulation of neurotransmitter uptake Bergmann glial cell differentiation positive regulation of glial cell proliferation long-term synaptic potentiation regulation of protein complex assembly regulation of chaperone-mediated autophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GFAP Polyclonal Antibody

GFAP Antibody (OPA1-06100) in IHC (F)

Product Details

OPA1-06100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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