Invitrogen

HSP70 Monoclonal Antibody (4G4)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

24 References

View all (60) HSP70 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

HSP70 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 21

HSP70 Antibody (MA3-009) in ICC/IF

Immunofluorescence analysis of HSP70 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSP70 Monoclonal Antibody(4G4) (Product # MA3-009) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dil... View More

HSP70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP70 Antibody in Immunoprecipitation (IP)

Product Image Gallery

HSP70 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in HeLa Cas9 cell line transduced with HSP70 Lentiviral sgRNA compared to control cell line using HSP70 Monoclonal Antibody (4G4) (Product # MA3-009). {KO}

Product Details

MA3-009

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Flow Cytometry (Flow)

1:100 - 1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

1:1,000

Neutralization (Neu)

View 3 publications 3 publications

Immunomicroscopy (IM)

View 1 publication 1 publication

Inhibition Assays (IA)

Assay-dependent

Cytotoxicity Assay (CT)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4G4

Immunogen

Human recombinant HSP70 over expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS, ascites

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325457

Product Specific Information

MA3-009 detects heat shock protein 70 kDa (HSP70) from mouse and human tissues. This antibody specifically detects HSP70 and does not cross-react with other members of the HSP70 family.

MA3-009 has been successfully used in Western blot, IF, blocking, FACS, inhibition assay, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 70 kDa protein representing HSP70 from K562 cell extract.

The MA3-009 antigen is recombinant human HSP70 over-expressed in E. coli. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human HSP70, a region that has been shown to be involved in stress-induced nucleolar localization.

Target Information

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Propagation of Distinct α-Synuclein Strains Within Human Reconstructed Neuronal Network and Associated Neuronal Dysfunctions.

Methods in molecular biology (Clifton, N.J.)

Gribaudo S,Bousset L,Courte J,Melki R,Peyrin JM,Perrier AL

MA3-009 was used in Immunocytochemistry-immunofluorescence to describe the methods used to reconstruct a cortico-cortical human neuronal network in microfluidic devices and how to take advantage of this cellular model to characterize (1) the prion-like properties of different α-Syn strains and (2) the neuronal dysfunctions and the alterations associated with the exposure to α-Syn strains or the nucleation of endogenous α-Syn protein in vitro.

Tue Nov 01 00:00:00 EDT 2022

Intravenous Immunoglobulin G Suppresses Heat Shock Protein (HSP)-70 Expression and Enhances the Activity of HSP90 and Proteasome Inhibitors.

Frontiers in immunology

Jones RJ,Singh RK,Shirazi F,Wan J,Wang H,Wang X,Ha MJ,Baljevic M,Kuiatse I,Davis RE,Orlowski RZ

MA3-009 was used in Western Blotting to support the possibility that anti-HSP70-1 IgG contained in IVIgG can inhibit myeloma and MCL growth by interfering with a novel mechanism involving uptake of exogenous HSP70-1 which then induces its own promoter.

Mon Apr 12 00:00:00 EDT 2021

Intravenous Immunoglobulin G Suppresses Heat Shock Protein (HSP)-70 Expression and Enhances the Activity of HSP90 and Proteasome Inhibitors.

Frontiers in immunology

Jones RJ,Singh RK,Shirazi F,Wan J,Wang H,Wang X,Ha MJ,Baljevic M,Kuiatse I,Davis RE,Orlowski RZ

Mon Apr 12 00:00:00 EDT 2021

The Potential of Nanobody-Targeted Photodynamic Therapy to Trigger Immune Responses.

Cancers

Beltrán Hernández I,Angelier ML,Del Buono D'Ondes T,Di Maggio A,Yu Y,Oliveira S

MA3-009 was used in Immunocytochemistry-immunoflourescence to explore the potential of NB-PDT to stimulate the immune system.

Wed Apr 15 00:00:00 EDT 2020

Targeting Extracellular Heat Shock Protein 70 Ameliorates Doxorubicin-Induced Heart Failure Through Resolution of Toll-Like Receptor 2-Mediated Myocardial Inflammation.

Journal of the American Heart Association

Liu P,Bao HY,Jin CC,Zhou JC,Hua F,Li K,Lv XX,Cui B,Hu ZW,Zhang XW

Published figure using HSP70 monoclonal antibody (Product # MA3-009) in Neutralization

Tue Oct 15 00:00:00 EDT 2019

Multiplex Biomarker Screening Assay for Urinary Extracellular Vesicles Study: A Targeted Label-Free Proteomic Approach.

Scientific reports

Chutipongtanate S,Greis KD

MA3-009 was used in Western Blotting to investigate the use of a multiplex biomarker screening assay focused on exosome-like vesicles for studying urinary extracellular vesicles.

Tue Oct 09 00:00:00 EDT 2018

Simultaneous Administration of Dexamethasone and Vitamin E Reversed Experimental Varicocele-induced Impact in testicular tissue in Rats; Correlation with Hsp70-2 Chaperone Expression.

International braz j urol : official journal of the Brazilian Society of Urology

Khosravanian H,Razi M,Farokhi F,Khosravanian N

Published figure using HSP70 monoclonal antibody (Product # MA3-009) in Western Blot

Tue Mar 29 00:00:00 EDT 2016

The bone marrow microenvironment enhances multiple myeloma progression by exosome-mediated activation of myeloid-derived suppressor cells.

Oncotarget

Wang J,De Veirman K,De Beule N,Maes K,De Bruyne E,Van Valckenborgh E,Vanderkerken K,Menu E

MA3-009 was used in Blocking experiments to investigate the effect of BMSC-derived exosomes on multiple myeloma BM cells, showing that multiple myeloma progression is enhanced through exosome-mediated activation of myeloid-derived suppressor cells.

Tue Dec 22 00:00:00 EST 2015

Testosterone and vitamin E administration up-regulated varicocele-reduced Hsp70-2 protein expression and ameliorated biochemical alterations.

Journal of assisted reproduction and genetics

Khosravanian N,Razi M,Farokhi F,Khosravanian H

Published figure using HSP70 monoclonal antibody (Product # MA3-009) in Western Blot

Sat Mar 01 00:00:00 EST 2014

Small-molecule proteostasis regulators for protein conformational diseases.

Nature chemical biology

Calamini B,Silva MC,Madoux F,Hutt DM,Khanna S,Chalfant MA,Saldanha SA,Hodder P,Tait BD,Garza D,Balch WE,Morimoto RI

MA3-009 was used in western blot to identify small-molecule proteostasis regulators and evaluate them

Sun Dec 25 00:00:00 EST 2011

Different rotavirus strains enter MA104 cells through different endocytic pathways: the role of clathrin-mediated endocytosis.

Journal of virology

Gutiérrez M,Isa P,Sánchez-San Martin C,Pérez-Vargas J,Espinosa R,Arias CF,López S

MA3-009 was used in blocking/activating experiment to investigate the mechanism for the rotavirus entry into host cells

Wed Sep 01 00:00:00 EDT 2010

Cytotoxic T lymphocytes carrying a pattern recognition protein Tag7 can detect evasive, HLA-negative but Hsp70-exposing tumor cells, thereby ensuring FasL/Fas-mediated contact killing.

Blood

Sashchenko LP,Dukhanina EA,Shatalov YV,Yashin DV,Lukyanova TI,Kabanova OD,Romanova EA,Khaidukov SV,Galkin AV,Gnuchev NV,Georgiev GP

MA3-009 was used in ELISA to investigate the role of a pattern recognition protein Tag7 in the FasL/Fas-mediated contact killing.

Sat Sep 15 00:00:00 EDT 2007

Cytotoxic T lymphocytes carrying a pattern recognition protein Tag7 can detect evasive, HLA-negative but Hsp70-exposing tumor cells, thereby ensuring FasL/Fas-mediated contact killing.

Blood

Sashchenko LP,Dukhanina EA,Shatalov YV,Yashin DV,Lukyanova TI,Kabanova OD,Romanova EA,Khaidukov SV,Galkin AV,Gnuchev NV,Georgiev GP

MA3-009 was used in ELISA to investigate the role of a pattern recognition protein Tag7 in the FasL/Fas-mediated contact killing.

Sat Sep 15 00:00:00 EDT 2007

Cytotoxic T lymphocytes carrying a pattern recognition protein Tag7 can detect evasive, HLA-negative but Hsp70-exposing tumor cells, thereby ensuring FasL/Fas-mediated contact killing.

Blood

Sashchenko LP,Dukhanina EA,Shatalov YV,Yashin DV,Lukyanova TI,Kabanova OD,Romanova EA,Khaidukov SV,Galkin AV,Gnuchev NV,Georgiev GP

MA3-009 was used in ELISA to investigate the role of a pattern recognition protein Tag7 in the FasL/Fas-mediated contact killing.

Sat Sep 15 00:00:00 EDT 2007

Cytotoxic T lymphocytes carrying a pattern recognition protein Tag7 can detect evasive, HLA-negative but Hsp70-exposing tumor cells, thereby ensuring FasL/Fas-mediated contact killing.

Blood

Sashchenko LP,Dukhanina EA,Shatalov YV,Yashin DV,Lukyanova TI,Kabanova OD,Romanova EA,Khaidukov SV,Galkin AV,Gnuchev NV,Georgiev GP

MA3-009 was used in ELISA to investigate the role of a pattern recognition protein Tag7 in the FasL/Fas-mediated contact killing.

Sat Sep 15 00:00:00 EDT 2007

Celastrols as inducers of the heat shock response and cytoprotection.

The Journal of biological chemistry

Westerheide SD,Bosman JD,Mbadugha BN,Kawahara TL,Matsumoto G,Kim S,Gu W,Devlin JP,Silverman RB,Morimoto RI

MA3-009 was used in western blot to investigate the role of the celastrols during the heat shock response and cytoprotection.

Fri Dec 31 00:00:00 EST 2004

Formation of nuclear stress granules involves HSF2 and coincides with the nucleolar localization of Hsp70.

Journal of cell science

Alastalo TP,Hellesuo M,Sandqvist A,Hietakangas V,Kallio M,Sistonen L

MA3-009 was used in western blot to demonstrate that subcellular distribution of Hsp70 contributes to HSF1-mediated heat-shock responses.

Mon Sep 01 00:00:00 EDT 2003

Contribution of heat shock proteins to cell protection from complement-mediated lysis.

International immunology

Fishelson Z,Hochman I,Greene LE,Eisenberg E

MA3-009 was used in flow cytometry to investigate the protective roles of heat shock proteins during complement-mediated lysis

Wed Aug 01 00:00:00 EDT 2001

Expression of inducible Hsp70 enhances the proliferation of MCF-7 breast cancer cells and protects against the cytotoxic effects of hyperthermia.

Cell stress & chaperones

Barnes JA,Dix DJ,Collins BW,Luft C,Allen JW

MA3-009 was used in western blot to study how inducible Hsp70 affects cell growth and protects against hyperthermia in MCF-7 cells

Mon Oct 01 00:00:00 EDT 2001

Differentiation lineage-specific expression of human heat shock transcription factor 2.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Pirkkala L,Alastalo TP,Nykanen P,Seppa L,Sistonen L

MA3-009 was used in western blot to investigate the functional properties of heat shock transcription factor 2 during differentiation

Tue Jun 01 00:00:00 EDT 1999

Definition of extracellular localized epitopes of Hsp70 involved in an NK immune response.

Cell stress & chaperones

Botzler C,Li G,Issels RD,Multhoff G

MA3-009 was used in western blot to identify the extracelluar part of human Hsp70 and its role in the induction of an NK-regulated immune response

Sun Mar 01 00:00:00 EST 1998

[In vivo gene transfection with heat shock protein 70 enhances myocardial tolerance to ischemia-reperfusion injury in rat].

Journal of cardiology

Suzuki K,Sawa Y,Kaneda Y,Ichihara H,Shirakura R,Matsuda H

MA3-009 was used in western blot to investigate the effect of HSP70 overexpression on myocardial protection in rat with ischemia-reperfusion injury

Sun Mar 01 00:00:00 EST 1998

In vivo gene transfection with heat shock protein 70 enhances myocardial tolerance to ischemia-reperfusion injury in rat.

The Journal of clinical investigation

Suzuki K,Sawa Y,Kaneda Y,Ichikawa H,Shirakura R,Matsuda H

MA3-009 was used in Western Blotting to indicate that overexpressed HSP70 plays a protective role in myocardial injury.

Tue Apr 01 00:00:00 EST 1997

Thioredoxin is transcriptionally induced upon activation of heat shock factor 2.

The Journal of biological chemistry

Leppä S,Pirkkala L,Chow SC,Eriksson JE,Sistonen L

MA3-009 was used in western blot to study the role of the activation of HSF2 during the regulation of TRX expression

Fri Nov 28 00:00:00 EST 1997

Overexpression of HSF2-beta inhibits hemin-induced heat shock gene expression and erythroid differentiation in K562 cells.

The Journal of biological chemistry

Leppä S,Pirkkala L,Saarento H,Sarge KD,Sistonen L

MA3-009 was used in western blot to study the role of the HSF2 isoforms during the hemin-mediated erythroid differentiation

Fri Jun 13 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: 68 kDa heat shock protein; 70 kilodalton heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein Li 5a; heat shock 10kDa protein 1-like; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; Heat shock 70 kDa protein 4; Heat shock 70 kDa-like protein 1; Heat shock 70-related protein APG-2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 2; heat shock 70kD protein 4; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; heat shock 70kDa protein 4; Heat shock protein; heat shock protein 70.1; Heat shock protein 70.2; heat shock protein cognate 70, testis; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 2; heat shock protein, 70 kDa 3; heat shock-induced protein; Heat shock-related 70 kDa protein 2; heat shock-related protein hsc70t; HEL S 103; HSP; Hsp68; HSP70 1; HSP70 1B; HSP70 2; HSP70 AceyltK77; hsp70 RY; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-Hom; HSP70.1; HSP70.1/HSP70.2; HSP70.3; HSP70RY; HSPA2; HSPA4; Hspa70; inducible heat shock protein 70; Spermatid-specific heat shock protein 70

View more View less

Gene Aliases: 70kDa; AI317151; APG-2; APG2; Hcp70.1; Hcp70.2; HEL-S-103; HEL-S-5a; HS24/P52; Hsc70t; Hsp110; hsp68; Hsp70; HSP70-1; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; HSP70-3; HSP70-HOM; HSP70.1; HSP70.2; Hsp70.3; Hsp70A1; HSP70A2; HSP70I; Hsp70RY; HSP70T; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA4; HSPH2; HSX70; hum70t; mKIAA4025; Msh5; RY

View more View less

UniProt ID: (Human) P0DMV8, (Human) P34931, (Human) P54652, (Human) P34932, (Mouse) Q61696, (Mouse) Q61689, (Mouse) P16627, (Mouse) P17156

View more View less

Entrez Gene ID: (Human) 3303, (Human) 3304, (Human) 3305, (Human) 3306, (Human) 3308, (Mouse) 193740, (Mouse) 15511, (Mouse) 15482, (Mouse) 15512, (Mouse) 15525

View more View less

Function(s)

virus receptor activity G-protein coupled receptor binding receptor binding protein binding ATP binding ATPase activity enzyme binding heat shock protein binding ubiquitin protein ligase binding ATPase activity, coupled histone deacetylase binding protein binding involved in protein folding unfolded protein binding C3HC4-type RING finger domain binding cadherin binding involved in cell-cell adhesion glycolipid binding protease binding double-stranded RNA binding poly(A) RNA binding protein N-terminus binding NF-kappaB binding nucleotide binding RNA polymerase II transcription factor binding protein complex binding activating transcription factor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

HSP70 Monoclonal Antibody (4G4)

HSP70 Antibody (MA3-009) in ICC/IF

Product Details

MA3-009

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Immunomicroscopy (IM)

Inhibition Assays (IA)

Cytotoxicity Assay (CT)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!