Invitrogen

SSEA5 Monoclonal Antibody (8E11), DyLight™ 488

SSEA5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

SSEA5 Antibody (MA1-144-D488) in ICC/IF

Immunofluorescent analysis of SSEA-5 (green) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 488-conjugated SSEA-5 monoclonal antibody (Product # MA1-144-D488) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. F-Actin (left panel, red) was stained with DyLight 554 Phalloidin (Product # 21834) and ... View More

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Product Details

MA1-144-D488

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

1:50-1:500

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

8E11

Immunogen

Undifferentiated H9 human embryonic stem cells

Conjugate

DyLight™ 488 DyLight™ 488 DyLight™ 488

Excitation/Emission Max

492/519 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536849

Product Specific Information

Immunofluorescence and flow cytometric analysis of MA1-144-D488 shows positive staining of SSEA-5 positive pluripotent cells and cultured human embryonal carcinoma NCCIT cells.

Target Information

Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-beta1-3GlcNAc-beta, which is characteristic of H-1 antigens. SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells and on the surface of induced pluripotent cells. SSEA-5 was found to proceed the loss of other known stem cell markers and the combination of SSEA-5 with either CD9 and CD90 or CD50 and CD200 was superior in identifying and eliminating rare undifferentiated human ES cells from differentiated cell populations than the use of other established stem cell markers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: SSEA-5

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