Invitrogen™
pEF6/V5-His TOPO™ TA Expression Kit
The pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with Read more
Catalog number K961020
Price (USD)/ 20 reactions
1,188.00
-
Price (USD)/ 20 reactions
1,188.00
pEF6/V5-His TOPO™ TA Expression Kit
Catalog numberK961020
Price (USD)/ 20 reactions
1,188.00
-
The pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO™ Cloning. The pEF6/V5-His-TOPO™ vector includes:
Enhancer/promoter elements from the human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells
• The Blasticidin resistance gene for rapid selection of stable mammalian cell lines
• A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody
• A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term)
• Antibody
Enhancer/promoter elements from the human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells
• The Blasticidin resistance gene for rapid selection of stable mammalian cell lines
• A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody
• A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term)
• Antibody
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
PromoterEF-1α
Product TypeTOPO TA Expression Kit
Selection Agent (Eukaryotic)Blasticidin
CleavageEK (Enterokinase) Recognition Site
Protein TagHis Tag (6x), V5 Epitope Tag
Cloning MethodTOPO-TA
Quantity20 reactions
VectorpEF, TOPO-TA Vectors
Product LineTOPO™
For Use With (Application)Constitutive Expression
Unit Size20 reactions
Contents & Storage
Each pEF6/V5-His TOPO™ TA Expression Kit contains two boxes. The TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pEF6/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control templates and primers, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Competent Cell box contains all of the reagents necessary for transformation, including twenty-one 50-μl aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store at -80°C. Guaranteed stable for 6 months when properly stored.