Invitrogen™
pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli and PureLink™ Kit
The pCR™8/GW/TOPO™ TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway™ recombination cloning vector platform. Our Read more
Catalog number K252002
Price (USD)/ 20 preps
712.00
-
Price (USD)/ 20 preps
712.00
pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli and PureLink™ Kit
Catalog numberK252002
Price (USD)/ 20 preps
712.00
-
The pCR™8/GW/TOPO™ TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway™ recombination cloning vector platform. Our pCR™8 TOPO™ TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector. And with our patented att sites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway™ recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.
The pCR™8/GW/TOPO™ TA Cloning Kit features:
• Fast growing E. coli that shortens cloning time
• Maximum convenience: includes plasmid miniprep kit
• att sites for rapid recombination into any Gateway™ destination vector
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
This kit includes One Shot™ Mach1™-T1R Chemically Competent E. coli and a PureLink™ Quick Plasmid Miniprep Kit.
The pCR™8/GW/TOPO™ TA Cloning Kit features:
• Fast growing E. coli that shortens cloning time
• Maximum convenience: includes plasmid miniprep kit
• att sites for rapid recombination into any Gateway™ destination vector
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
This kit includes One Shot™ Mach1™-T1R Chemically Competent E. coli and a PureLink™ Quick Plasmid Miniprep Kit.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning MethodTOPO™ with Gateway™
No. of Reactions20 preps
Product LineOne Shot™
Product TypeTA Cloning Kit
VectorpCR
Unit Size20 preps
Contents & Storage
pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers; One Shot™ TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink™ Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes. Store all components of the PureLink™ kit at room temperature. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.