Invitrogen™
PureLink™ HQ Mini Plasmid DNA Purification Kit
The PureLink HQ Mini Plasmid Purification Kit is designedfor the isolation of high-quality plasmid DNA that is suitablefor restriction enzyme Read more
Catalog number K210001
Price (USD)/ 100 preps
515.65
Online exclusive
556.00 Save 40.35 (7%)
-
Price (USD)/ 100 preps
515.65
Online exclusive
556.00 Save 40.35 (7%)
PureLink™ HQ Mini Plasmid DNA Purification Kit
Catalog numberK210001
Price (USD)/ 100 preps
515.65
Online exclusive
556.00 Save 40.35 (7%)
-
The PureLink HQ Mini Plasmid Purification Kit is designedfor the isolation of high-quality plasmid DNA that is suitablefor restriction enzyme digestion, PCR, sequencing, bacterialcell transformation, and mammalian cell transfection.Using the kit, plasmid DNA can be isolated from varyingamounts of bacterial cells.
The PureLink HQ Mini Plasmid Purification Kit has thefollowing advantages:
• Higher spin column capacity for plasmid DNA ascompared to other commercially available plasmidpurification systems
• Designed to isolate high-quality plasmid DNA in less thanan hour
• Minimal genomic DNA contamination of the purifiedsample
• Reliable performance of the purified DNA in downstreamapplications
Process overview
Bacterial cells are pelleted, resuspended, and lysed. Thelysate is then neutralized and conditions are adjusted forsubsequent binding. After clarification by centrifugation, thelysate is processed through the PureLink spin column. TheDNA binds to the silica-based membrane in the column, andimpurities are removed by a single wash step. The DNA isthen eluted in Elution Buffer or water.
The PureLink HQ Mini Plasmid Purification Kit has thefollowing advantages:
• Higher spin column capacity for plasmid DNA ascompared to other commercially available plasmidpurification systems
• Designed to isolate high-quality plasmid DNA in less thanan hour
• Minimal genomic DNA contamination of the purifiedsample
• Reliable performance of the purified DNA in downstreamapplications
Process overview
Bacterial cells are pelleted, resuspended, and lysed. Thelysate is then neutralized and conditions are adjusted forsubsequent binding. After clarification by centrifugation, thelysate is processed through the PureLink spin column. TheDNA binds to the silica-based membrane in the column, andimpurities are removed by a single wash step. The DNA isthen eluted in Elution Buffer or water.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeSpin Column
FormatSpin Column
Isolation TechnologySilica Spin Column
Sample TypeBacterial Culture
ScaleMini
Final Product TypePlasmid DNA
For Use With (Application)PCR, Transfection, Sequencing, Transformation, Cloning
High-throughput CompatibilityNot High-throughput Compatible (Manual)
No. of Reactions100 Preps
Prep Scale< 100 μg (Small-Scale) Plasmid DNA
Product LinePureLink™
Product TypePlasmid DNA Purification Kit
Quantity100 Preps
Shipping ConditionRoom Temperature
TargetPlasmid DNA
Unit Size100 preps
Contents & Storage
The PureLink HQ Mini Plasmid DNA Purification Kit includes sufficient PureLink columns, collection tubes, elution tubes, and reagents for 100 purifications. Store components at room temperature. Store Resuspension Buffer after addition of RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.