Tango™ HTR1A-bla U2OS Cells
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Thermo Scientific™

Tango™ HTR1A-bla U2OS Cells

Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a  Read more
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Catalog number K1857
Price (USD)/ 8 x 106 cells
10,580.00
-
Add to cart
Price (USD)/ 8 x 106 cells
10,580.00
Add to cart
Tango™ HTR1A-bla U2OS Cells
Catalog numberK1857
Price (USD)/ 8 x 106 cells
10,580.00
-
Add to cart

Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. Tango™ HTR1A-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of 5-CT (Figure 1). In addition, Tango™ HTR1A-bla U2OS cells have been tested for assay performance under variable conditions.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession NumberNM_000524
Assay EntryCell-based beta-arrestin recruitment
Cell StateDividing Cells
Cytoplasmic TailTail from AVPR2 GPCR
Detection MethodFluorescence
For Use With (Application)FRET
Gene SymbolHTR1A
Human Protein ClassG-Protein Coupled Receptor (GPCR)
Population StatusSingle Cell Clone Origin
Product LineTango™
Quantity8 x 106 Cells
ReadoutEnd Point
Target Entry5HT1A, HTR1A
Cell LineU2OS
System TypeTango™
Druggable TargetGPCR
Unit Size8 x 106 cells
Contents & Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• Tango™ HTR1A-UAS-bla CHO-K1 cells
• Tango™ HTR1A-UAS-bla CHO-K1 Assay Protocol
• Certificate of Analysis

The Tango™ HTR1A-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.