Thermo Scientific™
Tango™ GPR119-bla U2OS Cells
The Tango™ GPR119-bla U2OS cells contain the human G-protein Coupled Receptor 119 (GPR119) linked to a TEV protease site and Read more
Catalog number K1777
Price (USD)/ 8 x 106 cells
10,580.00
-
Price (USD)/ 8 x 106 cells
10,580.00
Tango™ GPR119-bla U2OS Cells
Catalog numberK1777
Price (USD)/ 8 x 106 cells
10,580.00
-
The Tango™ GPR119-bla U2OS cells contain the human G-protein Coupled Receptor 119 (GPR119) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ GPR119-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ GPR119-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession NumberNM_178471.1
Assay EntryCell-based beta-arrestin recruitment
Cell StateDividing Cells
Cytoplasmic TailNative Tail
Detection MethodFluorescence
Gene SymbolGPR119
Population StatusSingle Cell Clone Origin
Product LineGeneBLAzer™
Protein FamilyGPCR (G-Protein Coupled Receptors)
Quantity8 x 106 Cells
ReadoutEnd Point
Reporter GeneBLA (Beta-Lactamase)
Target EntryGPR119
Cell LineU2OS
System TypeTango™
Druggable TargetGPCR
Unit Size8 x 106 cells
Contents & Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.
Includes:
• Tango™ GPR119-bla U2OS cells
• Tango™ GPR119-bla U2OS Assay Protocol
• Certificate of Analysis
The Tango™ GPR119-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Includes:
• Tango™ GPR119-bla U2OS cells
• Tango™ GPR119-bla U2OS Assay Protocol
• Certificate of Analysis
The Tango™ GPR119-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.