GeneBLAzer™ T-REx™-G2A-NFAT-bla FreeStyle™ 293F Cells
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Thermo Scientific™

GeneBLAzer™ T-REx™-G2A-NFAT-bla FreeStyle™ 293F Cells

GeneBLAzer™ T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells contain the human GPR132 (G2A), (Accession No. NP_037477) stably integrated into the CellSensor™  Read more
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Catalog number K1749
Price (USD)/ 2 x 106 cells
10,580.00
-
Add to cart
Price (USD)/ 2 x 106 cells
10,580.00
Add to cart
GeneBLAzer™ T-REx™-G2A-NFAT-bla FreeStyle™ 293F Cells
Catalog numberK1749
Price (USD)/ 2 x 106 cells
10,580.00
-
Add to cart

GeneBLAzer™ T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells contain the human GPR132 (G2A), (Accession No. NP_037477) stably integrated into the CellSensor™ NFAT-bla Freestyle HEK 293F cell line. CellSensor™ NFAT-bla Freestyle HEK 293F cells (Cat. no. K1725) contain a beta-lactamase reporter gene under control of the NFAT Response Element.

The GeneBLAzer™ T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells are functionally validated for Z' and EC50 concentrations of dDAVP. In addition, GeneBLAzer™ GeneBLAzer™ T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

G2A is an orphan GPCR. Upon overexpession G2A is constitutively active. This is an assay for the orphan GPCR based upon constitutive activity. To use the assay to detect inverse agonists, high amounts of doxycycline should be added. To use the assay to detect agonists, low amounts of doxycycline should be added.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession NumberNM_013345
Assay EntryCell-based beta lactamase reporter gene
Cell StateDividing Cells
Detection MethodFluorescence
For Use With (Application)FRET
Gene SymbolG2A
Population StatusSingle Cell Clone Origin
Product LineGeneBLAzer™
Quantity2 x 106 Cells
Reporter GeneBLA (Beta-Lactamase)
Target EntryGPR132, G2A
Cell LineFreeStyle 293-F Cells
System TypeGeneBLAzer™
Druggable TargetGPCR
Unit Size2 x 106 cells
Contents & Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F cells
• GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F Assay Protocol
• Certificate of Analysis

The GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.