GeneBLAzer™ RARβ-UAS-bla HEK 293T Cells
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Thermo Scientific™

GeneBLAzer™ RARβ-UAS-bla HEK 293T Cells

RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fusedRead more
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Catalog number K1695
Price (USD)/ 2 x 106 cells
10,580.00
-
Add to cart
Price (USD)/ 2 x 106 cells
10,580.00
Add to cart
GeneBLAzer™ RARβ-UAS-bla HEK 293T Cells
Catalog numberK1695
Price (USD)/ 2 x 106 cells
10,580.00
-
Add to cart

RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of all-trans retinoic acid (ATRA). In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Alternate AgonistsDocosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Antagonistsnone
Assay EntryCell-based beta lactamase reporter gene
Cell StateDividing Cells
Detection MethodFluorescence
Druggable TargetNuclear Receptors
No. of Cells2 x 106
Primary Agonist9-cis-retinoic acid
Product LineGeneBLAzer™
Product TypeHEK 293T Cells
Reporter GeneBLA (Beta-Lactamase)
Target EntryRAR beta, NR1B2, RARB
Unit Size2 x 106 cells
Contents & Storage
GeneBLAzer™ Nuclear Receptor Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.