GeneBLAzer™ ADRB3 CHO-K1 DA Assay Kit
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Thermo Scientific™

GeneBLAzer™ ADRB3 CHO-K1 DA Assay Kit

The GeneBLAzer™ ADRB3 CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Beta 3 Receptor (ADRB3) (Accession No. NM_000025.1) stably  Read more
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Catalog number K1473
Price (USD)/ 1 Plate
1,172.00
-
Add to cart
Price (USD)/ 1 Plate
1,172.00
Add to cart
GeneBLAzer™ ADRB3 CHO-K1 DA Assay Kit
Catalog numberK1473
Price (USD)/ 1 Plate
1,172.00
-
Add to cart

The GeneBLAzer™ ADRB3 CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Beta 3 Receptor (ADRB3) (Accession No. NM_000025.1) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. No. K1129) contain a beta-lactamase (bla) reporter gene under control of the Cyclic AMP Response Element (CRE).

The ADRB3 CHO-K1 DA cells are functionally validated for Z'-factor and EC50 concentrations of Isoproterenol. In addition, ADRB3-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession NumberNM_000025.1
Assay EntryCell-based beta lactamase reporter gene
Cell StateDivision-Arrested Cells
Detection MethodFluorescence
Gene SymbolADRB3
Population StatusSingle Cell Clone Origin
Product LineGeneBLAzer™
Quantity1 Plate
Reporter GeneBLA (Beta-Lactamase)
Target EntryADRB3
Cell LineCHO-K1
System TypeGeneBLAzer™
Druggable TargetGPCR
Unit Size1 Plate
Contents & Storage
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• GeneBLAzer™ ADRB3 CHO-K1 DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• GeneBLAzer™ ADRB3 CHO-K1 Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The GeneBLAzer™ ADRB3 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.