Tango™ EDG4-bla U2OS Cells
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Thermo Scientific™

Tango™ EDG4-bla U2OS Cells

The Tango™ EDG4-bla U2OS cells contain the human Endothelial Differentiation Gene 4 (EDG4) linked to a TEV protease site and  Read more
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Catalog number K1442
Price (USD)/ 2 x 106 cells
10,580.00
-
Add to cart
Price (USD)/ 2 x 106 cells
10,580.00
Add to cart
Tango™ EDG4-bla U2OS Cells
Catalog numberK1442
Price (USD)/ 2 x 106 cells
10,580.00
-
Add to cart

The Tango™ EDG4-bla U2OS cells contain the human Endothelial Differentiation Gene 4 (EDG4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ EDG4-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of LPA (18:1). In addition, Tango™ EDG4-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession NumberNM_004720
Assay EntryCell-based beta-arrestin recruitment
Cell StateDividing Cells
Cytoplasmic TailTail from AVPR2 GPCR
Detection MethodFluorescence
Gene SymbolEDG4
Population StatusPool of Stable Cells
Product LineTango™
Quantity2 x 106 Cells
Reporter GeneBLA (Beta-Lactamase)
Target EntryEDG4
Cell LineU2OS
System TypeTango™
Druggable TargetGPCR
Unit Size2 x 106 cells
Contents & Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• Tango™ EDG4-bla U2OS cells
• Tango™ EDG4-bla U2OS Assay Protocol
• Certificate of Analysis

The Tango™ EDG4-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.