GeneBLAzer™ M1 CHO-K1 DA Assay Kit

This cell line is available as dividing cells by requesting a quote for K1230 from dividingcells@invitrogen.com

GeneBLAzer™ M1 CHO-K1 DA(Division Arrested) cells and M1-NFAT-bla CHO-K1 cells contain the human Acetylcholine (muscarinic) subtype 1 receptor (M1) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1078) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both M1 CHO-K1 DA cells and M1-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC₅₀ concentrations of carbachol, (Figure 1). In addition, M1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

The Musacarinic 1(M1) Receptor is localized principally in the cortical and hippocampal regions of the brain and has been implicated in various neurological conditions including depression, anxiety, and sleep disorders.